structure, function and dynamics interplay of RNA molecules

Deciphering the structure-function relationship of RNA: a complete guide

The interplay between dynamics, structure, and function of many RNA molecules is tied to essential biological processes, such as the regulation of gene expression and protein synthesis. However, modern imaging and biochemical methods often fail in explaining these relations. This application … Continue Reading

Application Note Enzymatic Activity Now Online

Enzymatic Activity and Inhibition Efficiency Application Note Now Online

The characterization of the enzymatic activity of proteins involved in the DNA machinery and the identification of their inhibitors are key for the development of a variety of treatments. It is essential to collect statistically-relevant data, which is typically achieved … Continue Reading

Motor Protein Application Note

Motor Protein Application Note Now Online

The cytoskeleton and its many molecular motors are major players in a variety of cellular functions. The interconnected networks of cytoskeletal filaments act as tracks directing molecular motor transport and provide structural support and organization to cells. One example of … Continue Reading

DNA Breathing

DNA Breathing Application Note Now Online

DNA breathing, also known as fraying, describes the spontaneous local conformational transitions occurring within double-stranded DNA (dsDNA). Due to the transient and spontaneous nature of this process, the study of real-time DNA breathing transitions at the single-molecule level requires the use of highly … Continue Reading

BIOCEV customer case story - Academy of Sciences of the Czech Republic, Faculty of Science and the First Faculty of Medicine of the Charles University in Prague

Customer Case Story BIOCEV Prague

In January 2016, LUMICKS’ first C-Trap™ Optical Tweezers-Fluorescence Microscopy instrument was successfully installed at the BIOCEV research center in the Czech Republic. BIOCEV is a research institute in the areas of biotechnology and biomedicine of both the Academy of Sciences … Continue Reading

cytoskeleton filament manipulation using optical tweezers fluorescence microscopy

Filament Manipulation Application Note Now Online

Protein filaments have been studied indirectly using various approaches of fluorescence microscopy in cells and tissues and more directly, using force spectroscopy on single filaments or filament bundles. LUMICKS’ C-Trap™ provides for a unique approach which combines the strength of … Continue Reading

Acoustic Force Spectroscopy Protein Unfolding Application Note high throughput

AFS Protein Unfolding Application Note Now Online

Single-molecule force spectroscopy (SMFS) technologies have become prominent as powerful tools for the investigation of the biomechanics associated with protein structure and for the study of protein unfolding pathways. However, current SMFS tools lack the ability to study multiple protein … Continue Reading

Multicolor detection of DNA overstretching

Multicolor Detection Application Note Now Online

Single-molecule force spectroscopy (SMFS) tools are widely used to study structural transitions of DNA during overstretching. However, as many of these tools only provide global information, the exact mechanisms that occur during these transitions remain unclear. Combining SMFS with visualization … Continue Reading

DNA Repair Visualization Application Note Now Online

DNA repair, the collection of highly-regulated mechanisms by which a cell identifies and repairs DNA damage, remains one of the most essential processes of human life. Without DNA repair mechanisms cells lose the ability to transcribe important regions of their … Continue Reading

Protein Unfolding Application Note Now Online

The biological function of many macromolecules such as proteins and RNA are tightly coupled to their conformation and their conformational dynamic. The correct folding of a protein into its native state is essential for its biological function. Misfolded or unfolded … Continue Reading