Protein Folding and Conformational Changes

Part of: Applications

Today’s scientific trends are racing towards smaller scales and experimentation that provides both structural and mechanistic insights. To decipher protein structure and function you need methods capable of studying how proteins fold correctly and undergo conformational changes to accomplish their biological function. We offer solutions that enable you to measure, manipulate and visualize protein folding and unfolding, with both high throughput and resolution.

Scientists can use optical tweezers to trap beads and catch a biomolecule, such as a protein, in between. The folding and unfolding of the protein can then be monitored by moving the beads while measuring the force and extension. The combination of optical tweezers with simultaneous multicolor fluorescence measurements (e.g. with FRET) allows correlating the global mechanical properties of the protein with the local structural properties. With optical tweezers – fluorescence microscopy you can:


  • Investigate protein folding and unfolding in real time, at the single molecule level and with high resolution
  • Measure highly detailed equilibrium dynamics over long periods of time to identify the protein (un)folding pathway and map the energy landscape
  • Correlate global mechanical properties of the protein with local structural properties
  • Perform experiments under biologically relevant conditions and highly crowded environments and link the in vitro experiment with the in vivo situation
  • Study the effect of small molecules and biologics in protein structure and dynamics.

Learn more about:
Optical tweezers - fluorescence microscopy working principle
Technology
Optical Tweezers and Fluorescence Microscopy

Scientists can use acoustic forces to manipulate, stretch and measure hundreds of single molecules at the same time. Multiple proteins can be tethered between a bead and a glass surface. Using Acoustic Force Spectroscopy (AFS) technology you can then apply controlled forces on all beads synchronously and probe the mechanical properties of each protein in parallel. With this highly parallel single-molecule method you can:


  • Investigate how multiple proteins fold and unfold in real time, at the single molecule level with high throughput
  • Measure equilibrium dynamics over long periods of time to identify the protein (un)folding pathway and map the energy landscape
  • Perform experiments under biologically relevant conditions and link the in vitro experiment with the in vivo situation
  • Study the effect of small molecules and biologics in protein structure, and
  • Receive large datasets containing many single-molecule measurements from a single experiment.

Learn more about:
Technology
Parallel Single-Molecule Force Spectroscopy
Optical Tweezers and Fluorescence Microscopy

Technology

The combination of optical tweezers and fluorescence microscopy allows for simultaneous manipulation and visualization of molecular interactions in real-time

Optical tweezers - fluorescence microscopy working principle
Solutions

C-Trap Optical Tweezers Fluorescence Microscopy
C-Trap™

Optical Tweezers and Fluorescence Microscopy

m-Trap™ Optical Tweezers
m-Trap™

Optical Tweezers

Parallel Single-Molecule Force Spectroscopy

Technology

A novel single-molecule and single-cell manipulation technology that allows the user to apply acoustic forces on multiple biomolecules and cells while tracking them in 3D with high accuracy.

Solutions

AFS™ Parallel Single-Molecule Force Spectroscopy
AFS™

Parallel Single-Molecule Force Spectroscopy

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