We are excited to announce that our latest application note, Real-time detection of kinase conformational changes in the presence of a small-molecule inhibitor, is available to download. The application note is perfect for protein research, as it showcases how you can use the C-Trap® Optical Tweezers – Fluorescence & Label-free system to measure transient protein conformations in detail.
We walk you through a case study, where our researchers examined the conformational properties of adenylate kinase (AdK) upon exposure to kinase inhibitor di(adenosine-5′) pentaphosphate (AP5A). We explain how the C-Trap can collect real-time data of single proteins and capture discrete conformational changes that reveal their functional properties. You will learn:
- How we tether a single protein to optically trapped beads.
- How we measure conformational changes of AdK upon exposure to small-molecule inhibitor AP5A.
- The workflow and features that make these types of experiments fast and straightforward, regardless of your previous experience level.
Download a copy of the application note here on the right to discover how you can perform real-time protein assessments with nanometer precision to extract the full conformation dynamics.