New application note shows how to study transient conformational changes of kinases using the C-Trap®

We are excited to announce that our latest application note, Real-time detection of kinase conformational changes in the presence of a small-molecule inhibitor, is available to download. The application note is perfect for protein research, as it showcases how you can use the C-Trap® Optical Tweezers – Fluorescence & Label-free system to measure transient protein conformations in detail.

We walk you through a case study, where our researchers examined the conformational properties of adenylate kinase (AdK) upon exposure to kinase inhibitor di(adenosine-5′) pentaphosphate (AP5A). We explain how the C-Trap can collect real-time data of single proteins and capture discrete conformational changes that reveal their functional properties. You will learn:

  • How we tether a single protein to optically trapped beads.
  • How we measure conformational changes of AdK upon exposure to small-molecule inhibitor AP5A.
  • The workflow and features that make these types of experiments fast and straightforward, regardless of your previous experience level.

Download a copy of the application note here on the right to discover how you can perform real-time protein assessments with nanometer precision to extract the full conformation dynamics.

Feel free to contact us if you want more information about the C-Trap® or our other instruments for single-molecule or single-cell research.

Want to learn more about this application and our solutions?

Optical Tweezers and Fluorescence Microscopy

Technology

The combination of optical tweezers and fluorescence microscopy allows for simultaneous manipulation and visualization of molecular interactions in real-time.

Solutions

C-Trap

Optical Tweezers and Fluorescence Microscopy

m-Trap

Optical Tweezers

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