Single-molecule study assesses how cohesin complexes bridge DNA molecules using C-Trap correlated optical tweezers with fluorescence microscopy

In a new study published in the journal Science Advances, Gutierrez-Escribano et al. studied the role of cohesin complexes in DNA tethering to gain a better understanding of sister chromatid cohesion. Using the C-Trap correlated optical tweezers with fluorescence microscopy, they … Read More

Study finds essential Cas9–DNA interaction downstream of PAM using a single-molecule approach

A new publication shows how the lab of Dr. Bo Sun used the m-Trap™ to analyze the interactions between a Cas9–gRNA complex and DNA. Using a single-molecule approach to unzip the target double-stranded DNA, the team showed interaction points on the … Continue Reading

Study establishes molecular forces responsible for condensation of ribonucleoprotein-RNA complexes

Researchers investigated the molecular driving forces underlying the condensation and decondensation of ribonucleoproteins using the C-Trap™. Specifically, they showed that sequence-dependent features of the RNA and ribonucleoprotein molecules partially dictate phase transitions. These findings can help scientists further understand the … Continue Reading

AFS

New study maps internal and external structures of red blood cell aggregates by combining AFS™ and 3D imaging

Researchers used acoustic force spectroscopy (AFS™) in combination with 3D imaging to induce clustering and rotation of red blood cells, enabling them to study the properties of cell aggregates. The new approach, which is based on radiation and stream forces, … Continue Reading

New study evaluates the dynamics behind viral assembly at the single-molecule level using both C-Trap™ and AFS™

Researchers used the C-Trap™ optical tweezers – fluorescence microscopy system and acoustic force spectroscopy (AFS) to study the processes underlying viral assembly. The study evaluated the capsid formation and DNA condensation of virus-like particles (VLPs) at the single-molecule level and … Continue Reading

New study resolves helicase dynamics and interactions during DNA replication using C-Trap™

Researchers from Rockefeller University used a C-Trap™ optical tweezers – fluorescence microscopy system to observe the behavior of the main component of the replisome – the eukaryotic CMG helicase – during DNA replication. The study offers exciting new insights into … Read More

Researchers used uFlux™ and optical tweezers to measure RecBCD activity

In a study performed at the Technion – Israel Institute of Technology, researchers used our  u-Flux™ laminar flow microfluidics system combined with optical tweezers to investigate the activity of the Escherichia coli enzyme RecBCD and its subunits. The paper titled “Synergy … Continue Reading

Researchers used C-Trap™ to measure energy landscapes and isomer stability in DNA origami

Researchers used C-Trap™ optical tweezers to study the energy landscapes associated with the formation of specific DNA origami structures, which is the folding of single-stranded DNA into pre-designed configurations. We congratulate all the authors involved in the study “Sites of … Read More

Two papers give new insights on protein droplets using LUMICKS’ C-Trap™

Two new papers published at Physical Review Letters and Nature Communications give new insights on protein droplets. Congratulations to all the authors! Jawerth et al. developed a method to investigate the frequency-dependent rheology and surface tension of protein droplets. They … Read More

New insights into Cas9 off-target activity

The paper “DNA stretching induces Cas9 off-target binding and cleavage” was just published in Nature Structural and Molecular Biology. Congratulations to all the authors! In this paper, scientists at the MRC London Institute of Medical Sciences and AstraZeneca studied how … Read More