Coordinated stimulation of RAD51 filament assembly and growth revealed with the C-Trap®

A recent study published in Molecular Cell used dynamic single-molecule analysis to evaluate the roles of specific proteins involved in the initiating steps of homologous recombination, including nucleation and growth of RAD51 filaments. Specifically, the researchers used the C-Trap® optical … Continue Reading

Measure Cell Mechanics with Acoustic Force Spectroscopy

Researchers study the viscoelasticity of human cells as a response to external cues using the high-throughput AFS®

A recent publication in Small introduces you to the value of acoustic force spectroscopy (AFS®) to study the viscoelastic properties of individual cells in high throughput. The authors describe how they used this “plug-and-play” instrument to measure mechanical cell properties … Continue Reading

Distal bridging between nucleosomes by polycomb complex PRC2 identified with the C-Trap®

A recent study published in PNAS used single-molecule force spectroscopy to examine unknown interaction mechanisms between polycomb repressive complex 2 (PRC2) and chromatin. The authors evaluated arrays of multiple nucleosomes using their C-Trap® optical tweezers system and identified distal nucleosome … Continue Reading

New study highlights interconvertible RecA–ssDNA states with the help of u-Flux™ microfluidics

A recent publication in FEBS Letters describes how the presence of ADP and ATP affects the structural transitions that single-stranded DNA (ssDNA) undergoes upon RecA-filament assembly. The researchers used the u-Flux™ Laminar Flow Microfluidics combined with optical tweezers to demonstrate … Continue Reading

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Force-dependent actin filament-binding by α-catenin recorded with the C-Trap®

A recent publication in the journal eLife describes a multidisciplinary study evaluating how the mechanical properties of actin filaments regulate the binding of associated proteins. Specifically, the researchers used the C-Trap® to demonstrate how applied forces on actin filaments enhance … Continue Reading

Researchers use the m-Trap® and C-Trap® to discover CRISPR-Cas9 properties that can help improve in vivo efficiency

A recent publication from the research lab of Dr. Bo Sun presents new findings in the field of gene editing that may help improve in vivo delivery of CRISPR-Cas9 complexes. The team investigated the DNA-binding properties of Staphylococcus aureus Cas9 … Continue Reading

Researchers investigate the effects of biomolecular droplet structure on fusion times using the C-Trap®

In a recent publication, researchers at the University of Illinois at Chicago used the C-Trap® to study the relationship between structural properties of biomolecular condensates and their fusion times. They found that fusion times, an indicator of condensate density, varies … Continue Reading

Electrostatic effects on vimentin stiffness demonstrated with the C-Trap®

A recently published study from the lab of Prof. Sarah Köster at the University of Göttingen investigated how electrostatic conditions influence the mechanical properties of intermediate filament vimentin, such as structural stability. The researchers assessed these features by stretching the … Continue Reading

Cell avidity is a superior predictor of CAR T-cell efficacy in myeloma than cytotoxicity – first published z-Movi® results

We are proud to announce the first publication showing results from cell–cell interaction measurements using the z-Movi® Cell Avidity Analyzer! In the study, Fernández de Larrea et al. demonstrate how cell avidity between CAR T cells and target multiple myeloma … Continue Reading

In vitro characterization of nucleosomes upon DNA unwrapping using the C-Trap®

A recent publication in the journal Scientific Reports describes a new method to create arrays of nucleosome positioning motifs. Through sequential Gibson Assembly reactions, the researchers managed to clone a series of sequence motifs, with high affinity for histone octamers, … Continue Reading