Exploring mitochondrial translation processes with the C-Trap®

This month, we find the C-Trap®  featured in Nature Communications in an article published by the lab of Dr. Joanna Rorbach in which they describe mitochondrial translation initiation. The study provides insight into a relatively unexplored, yet exciting field that … Continue Reading

Recent review highlights the impact of force spectroscopy and the C-Trap

We were happy to read last week that LUMICKS was recognized for our efforts in the field of dynamic single-molecule research! A recent review in ACS Publications examined the current advances in single-molecule research, highlighting the impact of force spectroscopy … Continue Reading

Single-molecule measurements with AFS® and optical tweezers reveal progressive assembly of viruses

The research lab of Dr. Gijs Wuite in the Netherlands recently published a single-molecule study in Science Advances, demonstrating the progressive self-assembly steps of viruses. Methods, such as optical tweezers and acoustic force spectroscopy (AFS®), enabled the researchers to study … Continue Reading

New insights into the initiation of nucleotide excision repair found using C-Trap

A recent publication in PNAS shows how the catalytically inactive alkyltransferase-like protein (ATL) scans and recognizes detrimental DNA alkylation lesions. The C-Trap® with correlated optical tweezers and confocal microscopy enabled researchers to investigate how ATL interacts with DNA to identify … Continue Reading

Screening of cancer targeting TCRs with the z-Movi®

With the quick development of new single-cell analysis technologies it is now possible to identify cancer-targeting T-Cell Receptors (TCRs) in a single experiment. A recent review published in Methods in Enzymology, presented the z-Movi® Cell Avidity Analyzer among the future … Continue Reading

New mechanism of helicase-induced DNA unwinding from nicks found with the C-Trap

A study published in eLife shows how replication protein A (RPA) activates helicase Bloom syndrome protein (BLM) to unwind double-stranded DNA (dsDNA) bidirectionally from nicks. The C-Trap® enabled the researchers to simultaneously measure and visualize the DNA–protein interactions in real … Continue Reading

Controlled ligand gradient through C-Trap facilitates DSM measurements

A publication in the journal Micromachines describes how researchers managed to control the concentrations of DNA-binding dyes in single-molecule optical tweezers experiments on DNA. They established a controlled concentration gradient with the multi-channel laminar microfluidics system integrated with their C-Trap® … Continue Reading

Study shows detailed properties of Ca2+ sensors during membrane fusion

A recent study in the Biophysical Journal shows how a Dutch research group measured and visualized dynamic interactions between membranes in the presence of Ca2+ sensors and synaptic inducers Syt1 and Doc2b. With the combined optical tweezers and confocal fluorescence … Continue Reading

A new DNA–protein coupling procedure improves stability for DSM analyses

A recent study in Communications Chemistry shows how a research lab at AMOLF in the Netherlands optimized DNA–protein coupling to study dynamic processes requiring high forces and long-term assessments. Using their C-Trap® Optical Tweezers – Fluorescence Microscopy system, the team … Continue Reading

Study shows how chaperone enzyme ClpB processively translocates polypeptide loops using C-Trap correlated optical tweezers and confocal microscopy

A new study published in Nature reports how researchers verified the intricate process of disaggregating entangled proteins by analyzing chaperone enzyme ClpB at a single-molecule level. The C-Trap® correlated optical tweezers and confocal microscopy enabled them to analyze how the … Continue Reading