Dynamic single-molecule analysis

Part of: Knowledge repository

Get a better understanding of dynamic single-molecule analysis, why is it important and relevant, and the working principle of our technology to measure and visualize biomolecular processes as they happen.

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Biological relevance

Drug research

Understand the underlying mechanisms of targets and the mode of action (MoA) of leads to accelerate drug research.

The role of forces in biology

Cells communicate in two primary methods: electrochemical signals (which dictate cell proliferation, adhesion, migration, and reproduction) and direct forces (such as DNA transduction, binding, or repair).

Technologies

correlated Optical Tweezers - Fluorescence Microscopy

Correlated optical tweezers with fluorescence microscopy

The combination of optical tweezers and fluorescence microscopy allows for simultaneous manipulation and visualization of molecular interactions in real-time.

What are optical tweezers?

Arthur Ashkin won the Nobel Prize in Physics “for the optical tweezers and their application to biological systems”. In essence, he discovered that light’s momentum could serve as an incredibly sensitive set of tweezers to catch and study biomolecules.

Correlated microscopy methods

IRM (label-free)

IRM allows you to visualize microtubules without the need for fluorescence labeling.

TIRF

Suitable for visualization on surfaces as it eliminates background fluorescence outside the focal plane.

STED Super Resolution

STED nanoscopy allows you to distinguish between individual DNA-binding proteins in close proximity.

Confocal

The confocal microscope emits light by a laser through a pinhole that allows a specific light wavelength to pass

Widefield

Widefield fluorescence microscopy illuminates the sample from below and uses light of frequencies that can excite fluorescent molecules associated to your sample. 

Resources

Webinars

Uncover a range of on-demand webinars delivered by our application scientists or invited speakers on a range of topics.

Applications notes

Read our latest application notes on your topic of interest…

Publications

Read the latest publications from LUMICKS instrument users here…

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Download our Cell Therapy (CAR-T, TCR, NK) applications deck