C-Trap™ G2

Best-in-class
Optical Tweezers – Fluorescence & Label-free Microscopy

C-Trap™ G2

Best-in-class
Optical Tweezers – Fluorescence & Label-free Microscopy

The C-Trap™ is the world’s first instrument that allows simultaneous manipulation and visualization of single-molecule interactions in real time.

It combines high resolution optical tweezers, fluorescence and label-free microscopy and an advanced microfluidics system in a truly integrated and correlated solution.

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What’s new in 2019?

The Surface Assay Toolkit is a unique assortment of new features that combines the best of surface imaging and optical tweezers into a fully correlated platform. It brings together optical tweezers with label-free microtubule detection using IRM, fluorescent TIRF and Widefield and introduces new performance features to ensure the most stable experiments at the surface.

Want to explore the latest capabilities of C-Trap?

New applications, new features, better specs, and more!

The C-Trap™ is the world’s first instrument that allows simultaneous manipulation and visualization of single-molecule interactions in real time.

It combines high resolution optical tweezers, fluorescence and label-free microscopy and an advanced microfluidics system in a truly integrated and correlated solution.

Jump to:

What’s new in 2019?

The Surface Assay Toolkit is a unique assortment of new features that combines the best of surface imaging and optical tweezers into a fully correlated platform. It brings together optical tweezers with label-free microtubule detection using IRM, fluorescent TIRF and Widefield and introduces new performance features to ensure the most stable experiments at the surface.

Want to explore the latest capabilities of C-Trap?

New applications, new features, better specs, and more!

How does it work…

In 2018 Arthur Ashkin won the Nobel Prize in Physics for his discovery that the momentum of light can be used as an incredibly sensitive set of “tweezers”.

By shooting a laser through a microscope, he created a highly focused beam of light strong enough to trap and hold in place objects such as plastic beads. These beads can be coated to stick to a variety of biomolecules, such as proteins, cytoskeleton filaments, DNA or RNA. Furthermore, the tiniest forces applied to these molecules can be measured as well, giving the world access to a tool not only capable of manipulating biomolecules but also capable of detecting what’s happening to them.

…and how can it be applied to my research?

Whether you are aiming to make novel discoveries in the life sciences or develop successful drug candidates, we work on a personal level to find the right solution for you. Browse our extended list of applications to discover how these technologies can benefit your research.

Don’t see your specific field represented? Drop us an e-mail to see what we can do!

Stable and precise sample manipulation

Different optical tweezers configurations and advanced manipulation features that allow you to get the most out of your sample.

Dual (2)
Optical Traps

For your typical assay in solution

Quadruple (4)
Optical Traps

Excellent for complex interactions

Single (1)
Optical Trap

For highly stable surface assays

Temperature Control

Perform your experiments under relevant conditions

Sample Stage

Ultra-precise measurements at the surface

Force Detection

Force sensing detectors with optional ultra-fast sensors

Stable and precise sample manipulation

Different optical tweezers configurations and advanced manipulation features that allow you to get the most out of your sample.

Dual (2) Optical Traps

For your typical assay in solution

Quadruple (4) Optical Traps

Excellent for complex interactions

Single (1) Optical Trap

For highly stable surface assays

Temperature Control

Perform your experiments under relevant conditions

Sample Stage

Ultra-precise measurements at the surface

Force Detection

Force sensing detectors with optional ultra-fast sensors

A wide variety of visualization capabilities

Choose and combine from a variety of imaging methods designed specifically to solve the needs of different experimental designs.

Confocal Fluorescence

The multicolor confocal fluorescence imaging allows the visualization of biological processes such as protein kinetics and (un)binding events on DNA. It also makes it possible to measure conformational changes of proteins by combining the C-Trap with FRET. With its fast 1D scanning capabilities it is suitable for constructs such as DNA or filaments.

STED Super Resolution

Upgrade to C-Trap SR for stimulated emission depletion (STED) nanoscopy to distinguish between individual DNA-binding proteins in close proximity. Perform experiments in highly crowded environments with unprecedented resolution (< 35 nm).

Comparison of 1D line trace (kymograph) between Confocal FLuorescence (left) and STED nanoscopy (right).

IRM

Label-free Imaging

Interference Reflection Microscopy (IRM) allows you to visualize biological structures in 3D without the need for fluorescence labeling. Its high signal-to-noise ratio lets you visualize dynamic cytoskeletal interactions with the highest contrast and temporal resolution.

IRM can be correlated to both Widefield and TIRF!

Widefield

Fluorescent Imaging in Solution

Use Widefield microscopy to image biomolecular processes in solution with high acquisition rates. Suitable for obtaining fast movies of trapped constructs at low protein concentrations.

Correlated Widefield IRM

Widefield can be correlated to IRM!

TIRF

Fluorescent Surface Imaging

Total Internal Reflection Fluorescence (TIRF) is the perfect fluorescence imaging method for visualization at the surface. It allows you to image biomolecules while eliminating background fluorescence outside the focal plane.

TIRF can be correlated to IRM!

Imaging Combinations

Choose confocal upgradable to STED for the highest resolution or combine label-free IRM with TIRF or Widefield to perform truly revolutionary experiments.

High throughput experiment workflow

Take a peek into our unique microfluidic & software workflow

Step 1

Load your sample

In Solution

Simply pipette your molecules into the syringes of the microfluidics system.

At the surface

Alternatively, prepare your sample in your custom-built flow cell.

Step 2

Assemble your assay

In Solution

Multiple adjacent laminar flow channels that do not mix (no physical barriers are involved). Use the traps to move between flows to pick up anything you like.

At the surface

Beads float in solution, while microtubules are at the surface. Bring them together using click-to-move optical traps and the new sample stage option.

Step 3

Perform your experiment

With our Bluelake software suite

With a click of a mouse, perform complex single-molecule experiments and gather simultaneous force, imaging, and instrument status data streams.

To make your experiments at the surface complete, the new Surface Assay Toolkit comes with new software features including active stabilization control, angle detection & vector forces as well as background rejection for IRM.

Easy flow cell navigation with user-created paths and positions

Visualization and interaction with the optical traps through live camera view

Click-to-move traps for navigating between beads and your sample

Applying vector forces along your microtubules

Done!

Get your data

After you set up the experiment, you are ready to go! Our intuitive software timeline interface for data storage automatically generates a structured view of the state of your experiments, allowing for fast and smooth navigation through multiple days of measurements.

The fully correlated data streams can be viewed, compared, and exported during or after your experiment. In addition, the extensive list of metadata is always streamed to disk, ensuring that you will never miss anything of value and will always be able to reproduce your experiments.

Bluelake is our extensive software suite for C-Trap and m-Trap systems. It’s a single package that features both intuitive controls for assembling and manipulating your assay, as well as a fully correlated data overview to ensure you can easily view and study the results of your experiment.

In addition, Bluelake is ready for automation features thanks to the ability to script almost every part of the system.

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