C-Trap® G2

Best-in-class
Optical Tweezers – Fluorescence & Label-free Microscopy

C-Trap® G2

Best-in-class
Optical Tweezers – Fluorescence & Label-free Microscopy

The C-Trap® is the world’s first instrument that allows simultaneous manipulation and visualization of single-molecule interactions in real time.

It combines high-resolution optical tweezers, fluorescence, and label-free microscopy, and an advanced microfluidics system in a truly integrated and correlated solution.

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What’s new?

Gain access to accessible, fast, and more reproducible than ever dynamic single-molecule (DSM) research using our ready-made consumables and tailored sample preparation services as well as a free, open-source user community scripting platform. Focus on your biological questions and generate the next wave of scientific discoveries in the least amount of time.

Testimonials and publications

How does it work…

In 2018 Arthur Ashkin won the Nobel Prize in Physics for his discovery that the momentum of light can be used as an incredibly sensitive set of “tweezers”.

By shooting a laser through a microscope, he created a highly focused beam of light strong enough to trap and hold in place objects such as plastic beads. These beads can be coated to stick to a variety of biomolecules, such as proteins, cytoskeleton filaments, DNA, or RNA. Furthermore, the tiniest forces applied to these molecules can be measured as well, giving the world access to a tool not only capable of manipulating biomolecules but also capable of detecting what’s happening to them.

…and how can it be applied to my research?

Whether you are aiming to make novel discoveries in the life sciences or develop successful drug candidates, we work on a personal level to find the right solution for you. Browse our extended list of applications to discover how these technologies can benefit your research.

Don’t see your specific field represented? Drop us an e-mail to see what we can do!

Stable and precise sample manipulation

Different optical tweezers configurations and advanced manipulation features allow you to get the most out of your sample.

Dual (2)
Optical Traps

For your typical assay in solution

C-trap dual trap

Quadruple (4)
Optical Traps

Excellent for complex interactions

C-trap Q trap

Single (1)
Optical Trap

For highly stable surface assays

C-trap Single trap

Temperature Control

Perform your experiments under relevant conditions

Sample
Stage

Ultra-precise measurements at the surface

Force Detection

Force sensing detectors with optional ultra-fast sensors

Stable and precise sample manipulation

Different optical tweezers configurations and advanced manipulation features that allow you to get the most out of your sample.

Dual (2) Optical Traps

For your typical assay in solution

Quadruple (4) Optical Traps

Excellent for complex interactions

Single (1) Optical Trap

For highly stable surface assays

Temperature Control

Perform your experiments under relevant conditions

Sample Stage

Ultra-precise measurements at the surface

Force Detection

Force sensing detectors with optional ultra-fast sensors

A wide variety of visualization capabilities

Choose and combine from a variety of imaging methods designed specifically to solve the needs of different experimental designs.

Confocal Fluorescence

The multicolor confocal fluorescence imaging allows the visualization of biological processes such as protein kinetics and (un)binding events on DNA. It also makes it possible to measure conformational changes of proteins by combining the C-Trap with FRET. With its fast 1D scanning capabilities it is suitable for constructs such as DNA or filaments.

STED Super Resolution

Upgrade to C-Trap SR for stimulated emission depletion (STED) nanoscopy to distinguish between individual DNA-binding proteins in close proximity. Perform experiments in highly crowded environments with unprecedented resolution (< 35 nm).

IRM

Label-free Imaging

Interference Reflection Microscopy (IRM) allows you to visualize biological structures in 3D without the need for fluorescence labeling. Its high signal-to-noise ratio lets you visualize dynamic cytoskeletal interactions with the highest contrast and temporal resolution.

IRM
IRM Visualisation

IRM can be correlated to both Widefield and TIRF!

Widefield

Fluorescent Imaging in Solution

Use Widefield microscopy to image biomolecular processes in solution with high acquisition rates. Suitable for obtaining fast movies of trapped constructs at low protein concentrations.

Widefield IRM
IRM Widefield visualisation

Widefield can be correlated to IRM!

TIRF

Fluorescent Surface Imaging

Total Internal Reflection Fluorescence (TIRF) is the perfect fluorescence imaging method for visualization at the surface. It allows you to image biomolecules while eliminating background fluorescence outside the focal plane.

TIRF

TIRF can be correlated to IRM!

Imaging Combinations

Choose confocal upgradable to STED for the highest resolution or combine label-free IRM with TIRF or Widefield to perform truly revolutionary experiments.

Choose confocal upgradable to STED for the highest resolution or combine label-free IRM with TIRF or Widefield to perform truly revolutionary experiments.

High throughput experiment workflow

Loading your sample and analysing your data in less than 30 minutes.

Fast and easy workflow of C-Trap experiments

The C-Trap system offers a fast workflow while its intuitive control, automation, and data analysis features ensure the highest ease of use for all users.

Watch the video below to see the workflow for a typical C-Trap experiment, show-cased on the example of Cas9-DNA binding. In just a few steps, you can go from setting up your assay to analyzing your data!

For more details on the individual steps, explore the tabs above.

Step 1

Load your sample into the flow

With fast and simple pipetting

For solution assays, load your samples and conditions into the syringes of the 5-channel u-Flux™ Laminar Flow Microfluidics system. Pipetting each reagent takes seconds thanks to the twist-and-lock syringe adaptor, with which you can quickly and easily refill individual syringes.

C-Trap fluidics
C-Trap Cover slip

Step 2

Assemble your assay without physical barriers

With our new nano stage option and using laminar flow microfluidics

For solution assays, you can seamlessly move your optical traps to gather reagents, move them between the 5 microfluidics lanes, and assemble your constructs.

For surface assays, double-click on a bead to trap it. You can easily locate your samples using, for example, IRM or fluorescence microscopy. The high-resolution piezo-controlled nanostage, allows you to accurately move between positions (x and y) to place the trapped bead at the end of the microtubule and start your experiment.

Workflow

In solution

Multiple adjacent laminar flow channels that do not mix (no physical barriers are involved). Use the traps to move between flows to pick up anything you like.

At the surface

Beads float in solution, while microtubules are at the surface. Bring them together using click-to-move optical traps and the new sample stage option.

Step 3

Perform and automate your experiments

With our Bluelake software suite

Bluelake™ is an intuitive user-friendly software suite containing programs that simplify, automate, and enhance single-molecule experiments and real-time data gathering with a mouse click. For example, use Bluelake to:

  • Assemble your samples and run your experiment in 80 seconds.
  • Extract 18 sets of data in only 30 minutes.

Done!

Organize and analyze your data

With a dedicated software suite

Generate a structured experiment overview with fast and smooth navigation through multiple days of measurements. View, compare and export your fully correlated data stream during or after the experiment. The dedicated software automatically stores all your metadata so you never lose valuable information and always have the option to reproduce your experiments.

Analyzing and repeating experiments has never been easier, as our analysis software comes with tutorials and sample notebooks that can serve as a scaffold for your own analyses.

Kymotracker is a data analysis package that automatically tracks individual fluorescent traces on a recorded kymograph, allowing you to easily quantify protein-binding events and evaluate them over time.

Easy and intuitive software package

Check how you can completely automate your experiments using our user-centered software workflow.

Intuitive controls

Quick and easy sample interfacing

The software gives you total control over your experiments. Whether you are visually navigating through the laminar flow cell on the screen or selecting the area you wish to scan through the bright-field camera, our approach puts you in the front seat.

Fully correlated data

Everything in a single timeline

Bluelake was designed to control all aspects of your system from a single interface. The trap positions, force data, bright-field, fluorescence microscopy, and label-free images use the same software package, which truly correlates the experiments.

Ready-made reagents, kits, and tailored sample preparation services

Use our newly-established biochemistry platform and internal expertise for the purification, labeling, and preparation of your reagents for optical trapping experiments. With our multidisciplinary expertise in molecular biology, biochemistry, and dynamic single-molecule analysis, we’ve created the most extensive selection of ready-made reagents, kits, and tailored services to prepare your samples.

 

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