Explore a range of publications within our dynamic single-molecule application field
- DNA–binding proteins
- Protein folding
- Cytoskeletal structure and transport
- Phase separation
- Mechanobiology
- Other applications
- Bi L. et al, The convergence of head-on DNA unwinding forks induces helicase oligomerization and activity transition, PNAS, 2022
- Bernacchia L. et al, Identification of the target and mode of action for the prokaryotic nucleotide excision repair inhibitor ATBC, Biosci Rep, 2022
- Leicher, R et al., Single-stranded nucleic acid binding and coacervation by linker histone H1, Nature Structural and Molecular Biology, 2022
- Aicart-Ramos A. et al.,Long DNA constructs to study helicases and nucleic acid translocases using optical tweezers, Methods in Enzymology, 2022
- Hormeno S. et al, Human HELB is a processive motor protein that catalyzes RPA clearance from single-stranded DNA, elife, 2022
- Chardon F. et al., CENP-B-mediated DNA loops regulate activity and stability of human centromeres, Molecular Cell, 2022
- Kono S. et al., Resolving the subtle details of human DNA alkyltransferase lesion search and repair mechanism by single-molecule studies, PNAS, 2022
- Morin, J. A. et al. Sequence-dependent surface condensation of a pioneer transcription factor on DNA. Nature Physics 2022
- Renger, R. et al.,Co-condensation of proteins with single- and double-stranded DNA, PNAS, 2022
- Anand, R. et al. HELQ is a dual-function DSB repair enzyme modulated by RPA and RAD51. Nature 2021
- Paul, B. et al. Mechanics of CRISPR-Cas12a and engineered variants on λ-DNA. Nucleic Acids Research 2021
- Hill, C. H. et al. Structural and molecular basis for Cardiovirus 2A protein as a viral gene expression switch. Nature Communications 2021
- Zhang, Q. et al. Efficient DNA interrogation of SpCas9 governed by its electrostatic interaction with DNA beyond the PAM and protospacer. Nucleic Acids Research 2021
- Buzón, P. et al. Virus self-assembly proceeds through contact-rich energy minima. Science Advances 2021
- Davis, R. B. et al. FUS Oncofusion Protein Condensates Recruit mSWI/SNF Chromatin Remodeler via Heterotypic Interactions Between Prion-like Domains. Protein Science 2021
- Lin, SN. et al. Direct visualization of the effect of DNA structure and ionic conditions on HU–DNA interactions. Scientific Reports 2021,
- Losito, M. et al. Cas12a Target Search and Cleavage on Force-Stretched DNA. PCCP 2021
- Newton, M. D. et al. A Minimal Load-and-Lock RuII Luminescent DNA Probe. Angew. Chem. Int. Ed 2021
- Backer, A. S. et al. Elucidating the Role of Topological Constraint on the Structure of Overstretched DNA Using Fluorescence Polarization Microscopy. J. Phys. Chem. B 2021
- de Asis Balaguer, F. et al. CTP promotes efficient ParB-dependent DNA condensation by facilitating one-dimensional diffusion from parS. eLife 2021
- Quail, T. et al. Force generation by protein–DNA co-condensation. Nature Physics 2021
- Belan, O. et al. Generation of versatile ss-dsDNA hybrid substrates for single-molecule analysis. STAR Protocols 2021
- Lin, X. et al. Cooperative DNA looping by PRC2 complexes. Nucleic Acids Research 2021
- Ye, S. et al. Proximal Single-Stranded RNA Destabilizes Human Telomerase RNA G-Quadruplex and Induces Its Distinct Conformers. J. Phys. Chem. Lett. 2021
- Sánchez, H. et al. DNA replication origins retain mobile licensing proteins. Nature Communications 2021
- Keenen, M. M. et al. HP1 proteins compact DNA into mechanically and positionally stable phase separated domains. eLife 2021
- Belan, O. et al. Single-molecule analysis reveals cooperative stimulation of Rad51 filament nucleation and growth by mediator proteins. Molecular Cell 2021
- Leicher, R. et al. Single-molecule and in silico dissection of the interaction between Polycomb repressive complex 2 and chromatin. PNAS 2020
- Zhang, S. et al. Dynamics of Staphylococcus aureus Cas9 in DNA target Association and Dissociation. EMBO Rep 2020
- Spakman, D. et al. Constructing arrays of nucleosome positioning sequences using Gibson Assembly for single-molecule studies. Scientific Reports 2020
- Khawaja, A. et al. Distinct pre-initiation steps in human mitochondrial translation. Nature Communications 2020
- Rill, N. et al. Alkyltransferase-like protein clusters scan DNA rapidly over long distances and recruit NER to alkyl-DNA lesions. PNAS 2020
- Qin, Z. et al. Human RPA activates BLM’s bidirectional DNA unwinding from a nick. eLife 2020
- Kretzer, B. et al. Single-Molecule Mechanics in Ligand Concentration Gradient. Micromachines 2020
- Zhang, Q. et al. The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation. Science Advances 2019
- Gutierrez-Escribano, P. et al. A conserved ATP- and Scc2/4-dependent activity for cohesin in tethering DNA molecules. Science Advances 2019
- Marchetti, M. et al. Real-Time Assembly of Viruslike Nucleocapsids Elucidated at the Single-Particle Level. Nano Letters 2019
- Wasserman, M. et al. Replication Fork Activation Is Enabled by a Single-Stranded DNA Gate in CMG Helicase. Cell 2019
- Zheng, Q. et al. Reversible histone glycation is associated with disease-related changes in chromatin architecture. Nature Communications 2019
- Newton, M. et al. DNA stretching induces Cas9 off-target activity. Nature Structural & Molecular Biology 2019
- Liu, J. et al. Minimalist design of polymer-oligopeptide hybrid as intrinsically disordered protein-mimicking scaffold for artificial membraneless organelle. ACS Cent. Sci. 2022
- Naqvi, M. et al. Protein chain collapse modulation and folding stimulation by GroEL-ES, Sci. Advances, 2022
- Zimmer, M. M. et al. The short isoform of the host antiviral protein ZAP acts as an inhibitor of SARS-CoV-2 programmed ribosomal frameshifting. Nature Communications 2021
- Wruck, F. et al. The ribosome modulates folding inside the ribosomal exit tunnel. Communications Biology 2021
- Maciuba, K. et al. Facile tethering of stable and unstable proteins for optical tweezers experiments. Biophysical Journal 2021
- Avellaneda, M. et al.Simultaneous sensing and imaging of individual biomolecular complexes enabled by modular DNA–protein coupling. Communications Chemistry 2020
- Avellaneda, M. et al.Processive extrusion of polypeptide loops by a Hsp100 disaggregase. Nature 2020
- Kosinski R. et al. Sites of high local frustration in DNA origami. Nature Communications 2019
- Schepers, A. V. et al. Multiscale mechanics and temporal evolution of vimentin intermediate filament networks. PNAS 2021
- Kučera, O. et al. Anillin propels myosin-independent constriction of actin rings. Nature Communications 2021
- Schaedel, L. et al. Vimentin Intermediate Filaments Stabilize Dynamic Microtubules by Direct Interactions. Nature Communications 2021
- Lam, A. J. et al. A Highly Conserved 310-Helix Within the Kinesin Motor Domain is Critical for Kinesin Function and Human Health. Science Advances 2021
- Budaitis, B. G. et al. Pathogenic Mutations in the Kinesin-3 Motor KIF1A Diminish Force Generation and Movement Through Allosteric Mechanisms. Journal of Cell Biology 2021
- Kraxner, J. et al. Post-Translational Modifications Soften Vimentin Intermediate Filaments. Nanoscale 2020
- Mei, L. et al. Molecular mechanism for direct actin force-sensing by α-catenin. eLife 2020
- Schepers, A. et al. Tuning intermediate filament mechanics by indirect and direct charge variations. Nanoscale 2020
- Lorenz, C. et al.Lateral Subunit Coupling Determines Intermediate Filament Mechanics. Physical Review Letters 2019
- Forsting, J. et al.Vimentin Intermediate Filaments Undergo Irreversible Conformational Changes during Cyclic Loading. Nano Letters 2019
- Block, J.et al. Viscoelastic properties of vimentin originate from nonequilibrium conformational changes. Science Advances 2018
- Block, J. et al. Nonlinear Loading-Rate-Dependent Force Response of Individual Vimentin Intermediate Filaments to Applied Strain. Physical Review Letters 2017
- Manjia Li. et al, Controlling synthetic membraneless organelles by a red-light-dependent singlet oxygen-generating protein, Nature Communication, 2022
- Joris Van Lindt et al., F/YGG-motif is an intrinsically disordered nucleic-acid binding motif, RNA biology, 2022
- Leicher, R et al., Single-stranded nucleic acid binding and coacervation by linker histone H1, Nature Structural and Molecular Biology, 2022
- Alshareedah, I. et al. Quantifying Viscosity and Surface Tension of Multi-Component Protein-Nucleic Acid Condensates. Biophysical Journal 2021
- Alshareedah, I. et al. Programmable viscoelasticity in protein-RNA condensates with disordered sticker-spacer polypeptides. Nature Communications 2021
- Ghosh, A. et al. Shear relaxation governs fusion dynamics of biomolecular condensates. Nature Communications, 2021
- Davis, R. B. et al. FUS Oncofusion Protein Condensates Recruit mSWI/SNF Chromatin Remodeler via Heterotypic Interactions Between Prion-like Domains. Protein Science 2021
- Rhine, K. et al. Single-molecule and ensemble methods to probe RNP nucleation and condensate properties. Methods 2021
- Kaur, T. et al. Sequence-encoded and Composition-dependent Protein-RNA Interactions Control Multiphasic Condensate Morphologies. Nature Communications 2021
- Jawerth, L. et al. Protein condensates as aging Maxwell fluids. Science 2020
- Ghosh, A. and Zhou, H.-X. Determinants for Fusion Speed of Biomolecular Droplets. The Journal of Physical Chemistry B, 2020
- Alshareedah, I. et al. Phase transition of RNA−protein complexes into ordered hollow condensates. PNAS 2020
- Alshareedah, I. et al. Interplay between Short-Range Attraction and Long-Range Repulsion Controls Reentrant Liquid Condensation of Ribonucleoprotein–RNA Complexes. Journal of the American Chemical Society 2019
- Gui, X., et al. Structural basis for reversible amyloids of hnRNPA1 elucidates their role in stress granule assembly. Nature Communications 2019
- Kaur, T. et al. Molecular Crowding Tunes Material States of Ribonucleoprotein Condensates. Biomolecules 2019
- Jawerth, L. et al. Salt-Dependent Rheology and Surface Tension of Protein Condensates Using Optical Traps. Physical Review Letters 2018
- Evers T. et al., Single-Cell Mechanical Characterization of Human Macrophages, Advanced Nanobiomed Research, 2022
- Sheikhhassani, V. et al., Single cell force spectroscopy of erythrocytes at physiological and febrile temperatures reveals mechano-modulatory effects of atorvastatin, Soft matter, 2022
- Evers, T. M. J. et al. Single-cell analysis reveals chemokine-mediated differential regulation of monocyte mechanics. iScience, 2022
- Vasse, G. F. et al. Single Cell Reactomics: Real‐Time Single‐Cell Activation Kinetics of Optically Trapped Macrophages. Small Methods 2021
- Sorkin, R. et al. Synaptotagmin-1 and Doc2b Exhibit Distinct Membrane-Remodeling Mechanisms. Biophysical Journal 2020
- Freitag, M. et al. Identification and correction of miscalibration artifacts based on force noise for optical tweezers experiments. J. Phys. Chem. 2021
- Dai, X. et al. Optical tweezers-controlled hotspot for sensitive and reproducible surface-enhanced Raman spectroscopy characterization of native protein structures. Nature Communications 2021
Our solution
The C-Trap® Optical Tweezers – Fluorescence & Label-free Microscopy is the world’s first instrument that allows simultaneous manipulation and visualization of single-molecule interactions in real time. It combines high resolution optical tweezers, fluorescence and label-free microscopy and an advanced microfluidics system in a truly integrated and correlated solution.
The C-Trap offers you a fast workflow to seamlessly catch and manipulate single molecules. The instrument measures their structural changes or interactions while you visualize them in teal time with high spatial and temporal resolution, ultimately offering you a complete and detailed picture of biomolecular properties and interactions
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