Confocal

Blog posts

New insights into the dynamics of initial replisome assembly uncovered by the C-Trap^®

In a recent article published in Nature Communications, Sanchez et al. successfully employed LUMICKS’ C-Trap® to discover new insights into the molecular dynamics of initial replisome formation. In a collaborative effort between Prof. Dekker (TU Delft) and Prof. Diffley (Francis Crick Institute) laboratories, the scientists uncovered that origin recognition complex…

j.yeh18 May 2021

Blog posts

Highly reproducible single-molecule analysis of transient structures of native proteins using m-Trap^® dual optical tweezers combined with Raman spectroscopy

A recent publication by Dai et al. in Nature Communications presents an optical tweezers-coupled Raman spectroscopy platform providing tuneable and reproducible surface-enhanced Raman spectroscopy (SERS) measurements. By combining a Raman microscope with the dual-trap optical tweezers and microfluidic flow chamber of the m-Trap®, the scientists were able to characterize three…

j.yeh8 April 2021

ROS activation in macrophages optical tweezers fluorescence microscopy

Blog posts

Early activation of single macrophages captured by the C-Trap^®

In a recent article published in Small Methods, researchers reveal a method that uniquely captures the early phase of oxidative stress response in macrophages. Employing the powerful combination of optical tweezers, confocal fluorescent microscopy, and microfluidics of the C-Trap®, they measured the real-time intracellular response of individual macrophages to a…

j.yeh11 March 2021

Blog posts

In vitro characterization of nucleosomes upon DNA unwrapping using the C-Trap^®

A recent publication in the journal Scientific Reports describes a new method to create arrays of nucleosome positioning motifs. Through sequential Gibson Assembly reactions, the researchers managed to clone a series of sequence motifs, with high affinity for histone octamers, into one DNA molecule. The approach is ideal to start…

j.yeh16 July 2020

Blog posts

Study shows how chaperone enzyme ClpB processively translocates polypeptide loops using C-Trap^® correlated optical tweezers and confocal microscopy

A new study published in Nature reports how researchers verified the intricate process of disaggregating entangled proteins by analyzing chaperone enzyme ClpB at a single-molecule level. The C-Trap® correlated optical tweezers and confocal microscopy enabled them to analyze how the member of the Hsp100 chaperone family translocates looped polypeptide substrates…

j.yeh13 February 2020

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