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New insights into Cas9 off-target activity

The paper “DNA stretching induces Cas9 off-target binding and cleavage” was just published in Nature Structural and Molecular Biology. Congratulations to all the authors! In this paper, scientists at the MRC London Institute of Medical Sciences and AstraZeneca studied how CRISPR-Cas9 differentiates between on-targets and off-targets. They used the C-Trap®…

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