CRISPR/Cas9 Archives

Blog posts

Study finds essential Cas9–DNA interaction downstream of PAM using a single-molecule approach

A new publication shows how the lab of Dr. Bo Sun used the m-Trap® to analyze the interactions between a Cas9–gRNA complex and DNA. Using a single-molecule approach to unzip the target double-stranded DNA, the team showed interaction points on the DNA that affected Cas9 binding and cleavage. These results can…

j.yeh21 November 2019

Blog posts

New insights into Cas9 off-target activity

The paper “DNA stretching induces Cas9 off-target binding and cleavage” was just published in Nature Structural and Molecular Biology. Congratulations to all the authors! In this paper, scientists at the MRC London Institute of Medical Sciences and AstraZeneca studied how CRISPR-Cas9 differentiates between on-targets and off-targets. They used the C-Trap®…

j.yeh8 March 2019

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CRISPR/Cas9 Archives - LUMICKS

Study finds essential Cas9–DNA interaction downstream of PAM using a single-molecule approach

New insights into Cas9 off-target activity

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CRISPR/Cas9 Archives - LUMICKS

Study finds essential Cas9–DNA interaction downstream of PAM using a single-molecule approach

New insights into Cas9 off-target activity

Recent Posts

Yearly Archive

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