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Confocal

What is confocal microscopy? The confocal microscope scans a laser beam through a sample to excite molecules that are in focus. The molecules then emit photons, which are measured by a detector. A pinhole in front of the detector blocks out-of-focus light and hence improves image quality. Confocal microscopy offers…
j.yeh
19 February 2021
correlated Optical Tweezers - Fluorescence Microscopy

Correlated optical tweezers with fluorescence microscopy

Correlative optical tweezers with fluorescence (CTFM) is a dynamic single-molecule technique that combines optical tweezers, fluorescence microscopy, and microfluidics into a fully integrated platform. It can be used to apply and measure forces while simultaneously visualizing individual molecules in real-time. With this technology it is possible to perform simultaneous manipulation,…
j.yeh
19 February 2021

Widefield

What is widefield microscopy? Similar to brightfield microscopy, widefield fluorescence microscopy illuminates the sample from below. The difference is in the light. Instead of a white light, fluorescence microscopy uses light of frequencies that can excite fluorescent molecules associated to your sample. With the widefield fluorescence microscopy technique, you illuminate…
j.yeh
19 February 2021

What are optical tweezers?

What are optical tweezers and what can they do? In 2018 Arthur Ashkin won the Nobel Prize in Physics "for the optical tweezers and their application to biological systems". In essence, he discovered that light's momentum could serve as an incredibly sensitive set of tweezers to catch and study biomolecules.By…
j.yeh
18 February 2021
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