Customer case study: Using cell avidity to explore the network of intercellular interactions of NK cells in the tumor microenvironment
Prof. Mark Lowdell
Professor of Cell & Tissue Therapy at University College London and Chief Scientific Officer of INmune Bio, Inc
About Prof. Lowdell and his research focus
The innate killing ability of NK cells serves as a compelling immunotherapeutic tool
In the second mechanism, NK cells bind to the “death receptor” that sits on the surface of the cancer cell inducing signals that lead to cancer cell apoptosis.
NK cell failure is a major cause of cancer relapse
Overcoming NK cell failure with INKmune™
Exploring the Network of Cellular Interactions of NK Cells in the Tumor Microenvironment
Watch a snippet of Mark Lowdell’s webinar on finding the synapse with microscopy. If you would like to download the complete webinar, click here.
Cell avidity analysis directly measures and quantifies the synaptic interaction
Using microscopical techniques, the team of prof. Mark Lowdell saw that the NK cells seemed to form a more stable synapse with the tumor cell when they were activated. Prof. Mark Lowdell and his team use the z-Movi® Cell Avidity Analyzer as a solution to directly measure and quantify the synaptic interaction.
With cell avidity measurements, the team can rapidly distinguish NK sensitive- from NK-resistant tumor cells by quantifying amount of NK cells bound to cancer cells. In their research studies, the binding between resting NK cells and NK sensitive- (K562) tumor cells depicted a higher avidity score, indicating the formation of a stronger immune synapse. On the other hand, the binding between NK cells and NK-resistant (SKOV3) tumor cells showed a significantly lower avidity score, indicating a much weaker binding compared to NK sensitive- (K562) tumor cells (Figure 1).