Precisely manipulating genetic material at the single molecule level is gaining importance across life sciences – and so do the tools that allow researchers to do exactly that. The C-Trap system combines single molecule fluorescence microscopy with optical tweezers to manipulate DNA, allowing researchers to directly observe and track molecular events as they occur. Designing and creating specific DNA constructs is crucial for maximizing the potential of single molecule studies. In this application note we introduce the powerful combination of cutting edge biochemistry and single-molecule visualization methods to increase throughput and maximize the results gained from each individual measurement.

Key Learning Points

• How the golden gate method enables the creation of unique DNA constructs with multiple defined sequences of interest
• How smart use of the golden gate assay accelerates single-molecule experiments
• How research on chromatin organization and transcription regulation can benefit from combining golden gate with dynamic single-molecule microscopy