Dynamic Single-Molecule analysis for drug discovery

Get all the information you need from your proteins in a single experiment

Target Validation

Hit Identification

Hit-to-lead

Lead Optimization

Scientists can study how proteins fold correctly and identify state conformational changes crucial for the protein’s function and regulation using Dynamic Single-Molecule (DSM) tools. Once, a target has been identified, it is possible to further study the influence of different pharmaceuticals on the protein activity, conformational changes, and dynamics to discover promising leads based on their MOA.

Study protein conformational changes in real-time at the single-molecule level

Scientists can use optical tweezers to trap beads and catch a protein in between. The folding and unfolding of the protein can then be monitored by moving the beads while measuring the force and extension. The combination of optical tweezers with simultaneous multicolor fluorescence measurements allows correlation of the global mechanical properties of the protein with the local structural properties.

C-Trap Protein Folding

The C-Trap™ Optical Tweezers – Fluorescence Microscopy system provides the ability to apply and measure force and extension of a protein target while simultaneously obtaining the fluorescence signals from e.g. FRET fluorophores. This allows one to identify the (in)active and intermediate states and resolve the interaction energies of proteins. All these together provide important insights into the protein’s functional mechanism. Because of the C-Trap’s unique microfluidics system, this can be done under different experimental conditions: in the presence or absence of (ant)agonists, co-factors and/or pharmaceuticals, within the same experiment.

Figure 1 shows the obtained equilibrium dynamics trace of calmodulin – a calcium-binding protein. The graph reveals that calmodulin switches between two major states, an open and a closed one, without a clear preference. We can resolve intermediate steps as calmodulin occasionally jumps to a third state for short periods of time.

1 Full-length CaM protein at 10 mM Ca2+ showing equilibrium dynamics between multiple states, represented by the dashed grey lines. Data is recorded at 50 kHz (grey line) and averaged at 200 Hz (red line). The histogram quantifies the most populated states in the inset (right panel) showing two peaks at 6.5 ± 0.1 pN and 7.8 ± 0.09 pN (mean ± standard deviation).

Sample courtesy of Prof. Carlos Bustamante at the University of California, Berkeley.

Peltz et al. (2016) Nature Communications
Lamichhane et al. (2015) PNAS
Gupta et al. (2015) Nature Communications

Want to learn more?

Want to learn more?

Excited about the possibilities but not yet up-to-speed with the technology? Our application development team can help explain how our instruments can benefit your pharmaceutical research. Leave your e-mail and we'll be in touch!

Take your research to the next level.

Get exclusive news on the latest products, single-molecule events and breakthrough science.

Newsletter pop up
By clicking the subscribe button you agree to LUMICKS’ privacy policy.