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G2

AFS

Parallel Single-Molecule
Force Spectroscopy

Acoustic Force Spectroscopy (AFS™) is a new single-molecule and single-cell manipulation method capable of applying acoustic forces on hundreds of biomolecules in parallel for precise experimentation with high throughput.

Discover the power of sound

The essence of the AFS™ technology lies in a glass microfluidic chip with a piezo element that generates resonant acoustic waves (ultrasound). These resonant acoustic waves are used to exert forces on micrometer-sized particles with a different density than the surrounding medium — e.g. a polystyrene microsphere or a living cell.

The AFS™ G2 brings novel features and improved performance, such as the new automated XY stage, the drift correction option, integrated temperature control, and an individual electronic fingerprint per chip. These developments increase the data quality and improve the ease-of-use for highly-multiplexed force spectroscopy measurements.

Sitters et al. (2015)
Nature Methods

AFS Stand-alone G2

The AFS Stand-alone package allows you to perform force spectroscopy measurements straight out of the box. Start your force spectroscopy experiments right away, without the need for additional imaging tools. With a compact inverted microscope, five AFS flow cells, an intuitive image analysis software and a powerful workstation, the AFS Stand-Alone is the perfect solution for performing multiplexed single-molecule and single-cell experiments.

AFS™ Parallel Single-Molecule Force Spectroscopy

Acoustic Forces

• Maximum applicable force of >200 pN (Ø4.5 μm silica spheres)
• Minium force incremental step of 2 fN
• Loading rates ranging from 10-4 pN/s to 103 pN/s

Workstation & Software

Powerful workstation including LabView-based open-source software capable of:
• Real-time parallel 2D tracking of 1500 microspheres at 25 Hz
• Real-time parallel 3D tracking of 400 microspheres at 25 Hz
• Real-time position accuracy of 2 nm (XY) and 4 nm (Z) at 25 Hz

G2 Features

• Motorized stage for precise and fast sample movement in XY
• Exchangeable 39x, 26x and 13x magnifications included
• Five flow cells featuring smart functionalities for improved throughput

AFS™ Module G2

In addition to the AFS™ Stand-alone microscope platform, LUMICKS offers the AFS™ Module. Combine parallel acoustic manipulation with your own imaging methods. The AFS Module
is designed to be combined with inverted (fluorescence) microscopes, both commercial and home-built.

Acoustic Forces

• Maximum applicable force of >200 pN (Ø4.5 μm silica spheres)
• Minium force incremental step of 2 fN
• Loading rates ranging from 10-4 pN/s to 103 pN/s

Workstation & Software

Workstation including LabView-based open-source software capable of:
• Constant forces
• Dynamic force ramps
• User-defined time varying force profiles

G2 Features

• Large variety of upgrades and functionalities
• Integration support and design for your commercial or home-built system
• Five flow cells featuring smart functionalities for improved throughput

AFS Module

Highlighted Features

The AFS instrument is a versatile tool which can be used to answer a broad spectrum of research questions. Here we highlight two enabling features and their applications.

40x-Objective-Field-of-View acoustic force spectrometry

40x Magnification

This 40x magnification is perfect for use with small particles or if a high tracking accuracy is required.

20x-Objective-Field-of-View

20x Magnification

This 20x magnification provides a nice balance between parallelism and tracking precision.

10x-Objective-Field-of-View

10x Magnification

This 10x magnification is ideal for highly parallel measurements where precision is not crucial.

Bead size = 4.5 μm. Images were taken on an AFS™ Stand-Alone system. All three objectives are included in a standard purchase.

Integrated Temperature Control

Studying cells and biomolecules often requires precise control over the environment, including thermal circumstances. AFS flow cells come with heating ability included, allowing you to perform experiments under physiological conditions and investigate temperature-dependent events. The new integrated temperature control feature allows users to manipulate cells or biomolecules under biologically-relevant conditions and investigate temperature-dependent events in single molecule experiments. The flow cell applies heat over the full surface with an absolute accuracy of 0.2°C up to 40°C and a closed loop stability of 0.1°C.

AFS G2 chip

Specifications Overview

A detailed overview of options & specifications


Features AFS Stand-alone AFS Module AFS Module with integration package (tracking software, z-stage, illumination, camera)
XY-Tracking
Z-Tracking
Force calibration
Inverted fluorescence microscopy compatibility ●*

Applications
Force-distance curves
Bond ruptures
Force application
  * If the AFS chip holder is integrated on an inverted (fluorescence) microscope the same functionalities and applications as the AFS Module apply.

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