Visit LUMICKS
at ASCB Cell Bio 2022

Our next reveal and customer discoveries at the

LUMICKS Tech Talk

4 December 2022, 12:15 PM - 1:00 PM - Theater 1, Exhibit Halls AB

Understanding the mechanism of action of biological processes requires connecting the structure of biomolecules such as proteins and DNA to their function. Without insights into the interplay of structure and function, it is impossible to understand diseases and discover potential cures at the most fundamental level. To obtain a true understanding of these molecular processes it is key to directly observe the dynamic mechanism at play, know how proteins assemble and work together, and be able to modulate the process.

Here, we present a new dynamic single-molecule technology that provides these key parts of information by visualizing and manipulating biological processes in real-time at the single-molecule level. Furthermore, we will show how this technology – previously only accessible for experts – now gives any scientist the possibility to prove molecular mechanisms by directly quantifying the dynamic behavior of DNA-binding proteins.

We present several examples in which our technology enhances the understanding of DNA repair mechanisms, chromatin structure and stability, as well as DNA replication, and DNA editing tools. We show these single-molecule data are processed in an easy, rapid, and intuitive way. Thus, DNA binding specificity at the base-pair level is directly reported, as well as binding constants from direct visualization of individual complexes interacting with DNA, and accurate quantification of enzymatic processivity and protein diffusion.