Publications

Explore a range of publications within our dynamic single-molecule application field

Ahlawat, V. et al. Protamine-Mediated Tangles Produce Extreme Deoxyribonucleic Acid Compaction, JACS 2024
Chatterjee, S. et al. Probing the mechanism of nick searching by LIG1 at the single-molecule level. Nucleic Acids Research 2024
Goluguri, R. et al. How to scan naked DNA using promiscuous recognition and no clamping: a model for pioneer transcription factors. Nucleic Acids Research 2024
Li, Y. et al. RPA transforms RNase H1 to a bidirectional exoribonuclease for processive RNA–DNA hybrid cleavage. Nature Communications 2024
Hengel, S. et al. The human Shu complex promotes RAD51 activity by modulating RPA dynamics on ssDNA. Nature Communications 2024
Chua, G. et al. Differential dynamics specify MeCP2 function at nucleosomes and methylated DNA. Nature Structural & Molecular Biology 2024
Alcón, P. et al. FANCD2–FANCI surveys DNA and recognizes double- to single-stranded junctions. Nature 2024
Kretzer, B. et al. TMPyP binding evokes a complex, tunable nanomechanical response in DNA. Nucleic Acids Research 2024
Lokanathan Balaji, S. et al. DNA binding and bridging by human CtIP in the healthy and diseased states. Nucleic Acids Research 2024
Vizjak, P. et al. ISWI catalyzes nucleosome sliding in condensed nucleosome arrays. Nature Structural & Molecular Biology 2024
Liu, Z. et al. A Biophysics Toolbox for Reliable Data Acquisition and Processing in Integrated Force–Confocal Fluorescence Microscopy. ACS Photonics 2024
Kabtiyal, P. et al. Localized Plasmonic Heating for Single-Molecule DNA Rupture Measurements in Optical Tweezers, Nanoscale 2024
Kim, J.M. et al., , Dynamic 1D Search and Processive Nucleosome Translocations by RSC and ISW2 Chromatin Remodelers. eLife 2024
Chappidi, N. et al., PARP1-DNA co-condensation drives DNA repair site assembly to prevent disjunction of broken DNA ends. Cell 2024
Sushil Pangeni et al., Rapid Long-distance Migration of RPA on Single Stranded DNA Occurs Through Intersegmental Transfer Utilizing Multivalent Interactions. JMB (TJ Ha, JHU) 2024
Schaich, M.A. et al., Single-molecule analysis of purified proteins and nuclear extracts: Insights from 8-oxoguanine glycosylase 1. DNA repair (Ben Van Houten, U Pittsburgh) 2024
Guo, L. et al., Joint Efforts of Replicative Helicase and SSB Ensure Inherent Replicative Tolerance of G-Quadruplex. Advanced Science 2023
Bernacchia, L. et al., Developing novel antimicrobials by combining cancer chemotherapeutics with bacterial DNA repair inhibitors. Plos Pathogens 2023
Ramirez, D. et al., De novo fabrication of custom-sequence plasmids for the synthesis of long DNA constructs with extrahelical features, Biophysical Journal (Nynke Dekker, TU Delft) 2023
Buzón, P. et al. The Histone Chaperones SET/TAF‐1β and NPM1 Exhibit Conserved Functionality in Nucleosome Remodeling and Histone Eviction in a Cytochrome c ‐Dependent Manner. Advanced Science (Wouter Roos, University of Groningen) 2023
Fazio, N. et al. E. coli RecB Nuclease Domain Regulates RecBCD Helicase Activity but not Single Stranded DNA Translocase Activity. Journal of Molecular Biology (Timothy M. Lohman, St. Louis)
Sharma, M et al. Assembly mechanism of the inflammasome sensor AIM2 revealed by single molecule analysis. Nature Communications (Eva De Alba, UC Merced) 2023
Sánchez, H. et al. A chromatinized origin reduces the mobility of ORC and MCM through interactions and spatial constraint. Nature Communications (Nynke Dekker, TU Delft)
Liu, C. et al. RNase H1 facilitates recombinase recruitment by degrading DNA–RNA hybrids during meiosis. Nucleic Acids Research (Bo Sun, ShanghaiTech)
Wan, L. et al. Unlicensed origin DNA melting by MCV and SV40 polyomavirus LT proteins is independent of ATP-dependent helicase activity. PNAS (Bennett Van Houten, University of Pittsburgh) 2023
Cai, T. et al. Single-Molecule Force Spectroscopy of Deoxyribonucleic Acid and Deoxyribonucleic Acid Polymerase Activity Impacted by Alkylating Agents. The Journal of Physical Chemistry Letters (Huizhong Xu, Soochow University) 2023
Ramírez Montero, D. et al. De novo fabrication of custom-sequence plasmids for the synthesis of long DNA constructs with extrahelical features. Biophysicsl Journal (Nynke Dekker, TU Delft)
Newton, M. et al. Negative DNA supercoiling induces genome-wide Cas9 off-target activity. Molecular Cell (David Rueda, Imperial College & Simon Boulton, Francis Crick Institute)
Belan, O. et al. Visualization of direct and diffusion-assisted RAD51 nucleation by full-length human BRCA2 protein. Molecular Cell (David Rueda, Imperial College & Simon Boulton, Francis Crick Institute)
Greenhough L. A. et al, Structure and function of the RAD51BCD-XRCC2 tumour suppressor complex, Nature, (Simon Boulton & Steve West, Crick) 2023
Tang, M. et al. Establishment of dsDNA-dsDNA interactions by the condensin complex. Molecular Cell (Maxim Molodtsov, Francis Crick Instuitute)
Daniel Ramírez Montero, Nucleotide binding halts diffusion of the eukaryotic replicative helicase during activation Nature Communications, (Nynke Dekker, TU Delft) 2023
Kacey N.Mersch “Helicase” activity promoted through dynamic interactions between a ssDNA translocase and a diffusing SSB protein. PNAS (Timothy M. Loham, St. Louis) 2023
Ling Wang et. al., Head-on and co-directional RNA polymerase collisions orchestrate bidirectional transcription termination. Molecular Cell (Shixin Liu, Rockefeller University) 2023
Schaich M. et al., Single-molecule analysis of DNA-binding proteins from nuclear extracts (SMADNE). NAR (Ben Van Houten, University of Pittsburgh) 2023
Tanasie et al., Stabilization of DNA fork junctions by Smc5/6 complexes revealed by single-molecule imaging. Cell Reports (Johannes Stigler, LMU & Luis Aragon, Imperial College London) 2022
McCauley, M. et al., Human FACT subunits coordinate to catalyze both disassembly and reassembly of nucleosomes. Cell Reports (Mark Williams, Northeastern University) 2022
Jang S. et al., Cooperative interaction between AAG and UV-DDB in the removal of modified bases. NAR (Ben Van Houten, University of Pittsburgh) 2022
Belan O. et al., POLQ seals post-replicative ssDNA gaps to maintain genome stability in BRCA-deficient cancer cells. Molecular Cell (Simon Boulton, Crick Institute) 2022
Spakman et al., PICH acts as a force dependent nucleosome remodeler. Nature communications (Gijs Wuite, VU) 2022
J.Chang et al., Smc5/6’s multifaceted DNA binding capacities stabilize branched DNA structures. Nature Communication (Shixin Liu, Rockefeller University) 2022
Li et al., Origin recognition complex harbors an intrinsic nucleosome remodeling activity. PNAS (Shixin Liu, Rockefeller University) 2022
Kaczmarczyk A. et al.  Search and processing of Holliday junctions within long DNA by junction-resolving enzymes. Nature Communication (David Rueda, Imperial College) 2022
Carcamo, C. C. et al. ATP Binding Facilitates Target Search of SWR1 Chromatin Remodeler by Promoting One-Dimensional Diffusion on DNA. eLife (Taekjip Ha, Johns Hopkins University) 2022
Kuppa, et al. Rtt105 regulates RPA function by configurationally stapling the flexible domains. Nature Communications. (Edwin Antony and Taekjip Ha, Johns Hopkins University) 202
Li, S. et al. Nucleosome-directed replication origin licensing independent of a consensus DNA sequence . Nature Communications. (Shixin Liu, Rockefeller University) 2022
Lijuan, Guo. et al. Stochastically multimerized ParB orchestrates DNA assembly as unveiled by single-molecule analysis. Nucleic Acid Research. (Bo Sun, ShanghaiTech) 2022
Gien, H. et al. HIV-1 Nucleocapsid Protein Binds Double-Stranded DNA in Multiple Modes to Regulate Compaction and Capsid Uncoating. Viruses (Mark Williams, Northeastern University) 2022
Bi L. et al, The convergence of head-on DNA unwinding forks induces helicase oligomerization and activity transition, PNAS  (Bo Sun, ShanghaiTech) 2022
Kono S. et al., Resolving the subtle details of human DNA alkyltransferase lesion search and repair mechanism by single-molecule studies. PNAS, (Ingrid Tessmer, Würzburg) 2022
Bernacchia L. et al, Identification of the target and mode of action for the prokaryotic nucleotide excision repair inhibitor ATBC, Biosci Rep (Niel Kad, Kent University) 2022
Morin, J. A. et al. Sequence-dependent surface condensation of a pioneer transcription factor on DNA. Nature Physics (Stephan Grill, TU Dresden) 2022
Renger, R. et al.,Co-condensation of proteins with single- and double-stranded DNA. PNAS (Stephan Grill, TU Dresden) 2022
Hormeno S. et al, Human HELB is a processive motor protein that catalyzes RPA clearance from single-stranded DNA. Elife (Moreno, CNB, Madrid) 2022
Chardon F. et al., CENP-B-mediated DNA loops regulate activity and stability of human centromeres. Molecular Cell (Fachinetti, Institute Curie) 2022
Sánchez, H. et al. DNA replication origins retain mobile licensing proteins. Nature Communications (Nynke Dekker, TU Delft) 2021
Losito, M. et al. Cas12a Target Search and Cleavage on Force-Stretched DNA. PCCP (David Rueda, Imperial College London) 2021
Anand, R. et al. HELQ is a dual-function DSB repair enzyme modulated by RPA and RAD51. Nature (Simon Boulton, Crick Institute) 2021
Paul, B. et al. Mechanics of CRISPR-Cas12a and engineered variants on λ-DNA. Nucleic Acids Research (Guillermo Montoya, University of Copenhagen) 2021
Hill, C. H. et al. Structural and molecular basis for Cardiovirus 2A protein as a viral gene expression switch. Nature Communications (Neva Caliskan, Helmholtz Institute for RNA-based infection research (HIRI), University of Würzburg & Ian Brierly, University of Cambridge) 2021
Zhang, Q. et al. Efficient DNA interrogation of SpCas9 governed by its electrostatic interaction with DNA beyond the PAM and protospacer. Nucleic Acids Research (Bo Sun, ShanghaiTech) 2021
Buzón, P. et al. Virus self-assembly proceeds through contact-rich energy minima. Science Advances (Gijs Wuite, VU University Amsterdam & Wouter Roos, Rijksuniversiteit Groningen) 2021
Backer, A. S. et al. Elucidating the Role of Topological Constraint on the Structure of Overstretched DNA Using Fluorescence Polarization Microscopy . J. Phys. Chem. B (Gijs Wuite, VU University Amsterdam) 2021
de Asis Balaguer, F. et al. CTP promotes efficient ParB-dependent DNA condensation by facilitating one-dimensional diffusion from parS. eLife (Fernando Moreno-Herrero, Centro Nacional de Biotecnología) 2021
Quail, T. et al. Force generation by protein–DNA co-condensation. Nature Physics (Stephan Grill, TU Dresden) 2021
Belan, O. et al. Generation of versatile ss-dsDNA hybrid substrates for single-molecule analysis. STAR Protocols (David Rueda, Imperial College London) 2021
Lin, X. et al. Cooperative DNA looping by PRC2 complexes. Nucleic Acids Research (Shixin Liu, Rockefeller University) 2021
Ye, S. et al. Proximal Single-Stranded RNA Destabilizes Human Telomerase RNA G-Quadruplex and Induces Its Distinct Conformers . J. Phys. Chem. Lett. (Bo Sun, ShanghaiTech) 2021
Keenen, M. M. et al. HP1 proteins compact DNA into mechanically and positionally stable phase separated domains. eLife (Sy Redding, University of California San Francisco) 2021
Belan, O. et al. Single-molecule analysis reveals cooperative stimulation of Rad51 filament nucleation and growth by mediator proteins. Molecular Cell (David Rueda, Imperial College London) 2021
Zhang, S. et al. Dynamics of Staphylococcus aureus Cas9 in DNA target Association and Dissociation. EMBO Rep (Bo Sun, ShanghaiTech) 2020
Spakman, D. et al. Constructing arrays of nucleosome positioning sequences using Gibson Assembly for single-molecule studies. Scientific Reports (Gijs Wuite, VU University Amsterdam) 2020
Khawaja, A. et al. Distinct pre-initiation steps in human mitochondrial translation. Nature Communications (Joanna Rorbach, Karolinska Institute) 2020
Rill, N. et al. Alkyltransferase-like protein clusters scan DNA rapidly over long distances and recruit NER to alkyl-DNA lesions. PNAS (Ingrid Tessmer, University of Würzburg) 2020
Qin, Z. et al. Human RPA activates BLM’s bidirectional DNA unwinding from a nick. eLife (Bo Sun, ShanghaiTech) 2020
Kretzer, B. et al. Single-Molecule Mechanics in Ligand Concentration Gradient. Micromachines (Miklós Kellermayer, Semmelweis University) 2020
Leicher, R. et al. Single-molecule and in silico dissection of the interaction between Polycomb repressive complex 2 and chromatin. PNAS (Shixin Liu, Rockefeller University) 2019
Marchetti, M. et al.Real-Time Assembly of Viruslike Nucleocapsids Elucidated at the Single-Particle Level. Nano Letters (Gijs Wuite, VU University Amsterdam & Wouter Roos, Rijksuniversiteit Groningen) 2019
Wasserman, M. et al.Replication Fork Activation Is Enabled by a Single-Stranded DNA Gate in CMG Helicase. Cell (Shixin Liu & Michael O’Donnel, Rockefeller University) 2019
Kosinski R. et al. Sites of high local frustration in DNA origami. Nature Communications (Barbara Saccà, University of Essen-Duisburg) 2019
Zheng, Q. et al. Reversible histone glycation is associated with disease-related changes in chromatin architecture. Nature Communications (Shixin Liu, Rockefeller University and Yael David MSKCC) 2019
Newton, M. et al. DNA stretching induces Cas9 off-target activity. Nature Structural & Molecular Biology (David Rueda, Imperial College London) 2019

O’Brien, B. M. et al. Stick-slip unfolding favors self-association of expanded HTT mRNA. Nat Commun 2024
Walbrun, A. et al. Single-molecule force spectroscopy of toehold-mediated strand displacement. Nat Commun 2024
Litschel, T. et al. Membrane-induced 2D phase separation of the focal adhesion protein talin. Nat Commun 2024
Van Der Sleen, L. et al. Probing the stability and interdomain interactions in the ABC transporter OpuA using single-molecule optical tweezers. Cell Reports 2024
Momčilo Gavrilov et al. Free-energy measuring nanopore device. Physical Review E (Taekjip Ha, Johns Hopkins University) 2023
Mondol, T. et al. Aha1 regulates Hsp90’s conformation and function in a stoichiometry-dependent way. Biophysical Journal (Kasia Tych, University of Groningen) 2023
Wang, H. et al. Mechanistic Insights into the Folding Mechanism of Region V in Ice-Binding Protein Secreted by Marinomonas primoryensi Revealed by Single-Molecule Force Spectroscopy. Langmuir (Chunguang Hu, Tanjin University)
Scalvini, B. et al. Cytosolic Interactome Protects Against Protein Unfolding in a Single Molecule Experiment. Advanced Biology (Alireza Mashagi, Leiden University)
Sadhanasatish, T et al. A molecular optomechanics approach reveals functional relevance of force transduction across talin and desmoplakin. Science Advances (Matthias Rief, TUM) 2023
Pekarek L. et al., Cis-mediated interactions of the SARS-CoV-2 frameshift RNA alter its conformations and affect function. NAR (Neva Caliskan, HIRI) 2022
Freitag M. et al., Single-molecule experiments reveal the elbow as an essential folding guide in SMC coiled-coil arms. Biophysical Journal (Johannes Stigler, LMU) 2022
Walker, S. D. et al, The activated ClpP peptidase forcefully grips a protein substrate. Biophysical Journal (Adrian Olivares, Vanderbilt University) 2022
Buck, S. et al. POTATO: An automated pipeline for batch analysis of optical tweezers data. Biophysical Journal (Neva Caliskan, Helmholtz Institute for RNA-based infection research (HIRI), University of Würzburg) 2022
Liu, J. et al. Minimalist design of polymer-oligopeptide hybrid as intrinsically disordered protein-mimicking scaffold for artificial membraneless organelle. ACS Cent. Sci. (Jinqing Huang, Hong Kong University) 2022
Naqvi, M. et al.  Protein chain collapse modulation and folding stimulation by GroEL-ES, Sci. Advances (Sander Tans, AMOLF) 2022
Pekarek L et al., Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation, JoVE (Neva Caliskan, HIRI) 2022
Zimmer, M. M. et al. The short isoform of the host antiviral protein ZAP acts as an inhibitor of SARS-CoV-2 programmed ribosomal frameshifting. Nature Communications (Neva Caliskan, Helmholtz Institute for RNA-based infection research (HIRI), University of Würzburg) 2021
Maciuba, K. et al. Facile tethering of stable and unstable proteins for optical tweezers experiments. Biophysical Journal (Christian Kaiser , Johns Hopkins University) 2021
Wruck, F. et al. The ribosome modulates folding inside the ribosomal exit tunnel. Communications Biology (Sander Tans, AMOLF) 2021
Avellaneda, M. et al.Simultaneous sensing and imaging of individual biomolecular complexes enabled by modular DNA–protein coupling. Communications Chemistry (Sander Tans, AMOLF) 2020
Avellaneda, M. et al.Processive extrusion of polypeptide loops by a Hsp100 disaggregase. Nature (Sander Tans, AMOLF) 2020

Liu, X. et al. Kinesin-14 HSET and KlpA are non-processive microtubule motors with load-dependent power strokes. Nat. Commun. 2024
Bensel, B.M. et al. Kinesin-1-transported liposomes prefer to go straight in 3D microtubule intersections by a mechanism shared by other molecular motors. PNAS 2024
Muir, K. W. et al. Structural mechanism of outer kinetochore Dam1-Ndc80 complex assembly on microtubules. Science (David Barford, MRC-LMB Cambridge) 2023
Schepers, A. V. et al. Multiscale mechanics and temporal evolution of vimentin intermediate filament networks. PNAS, (Sarah Köster, University of Göttingen) 2021
Kučera, O. et al. Anillin propels myosin-independent constriction of actin rings. Nature Communications (Zdenek Lansky, BIOCEV) 2021
Schaedel, L. et al. Vimentin Intermediate Filaments Stabilize Dynamic Microtubules by Direct Interactions. Nature Communications, (Sarah Köster, University of Göttingen) 2021
Lam, A. J. et al. A Highly Conserved 310-Helix Within the Kinesin Motor Domain is Critical for Kinesin Function and Human Health. Science Advances (Arne Gennerich, Albert Einstein College of Medicine) 2021
Budaitis, B. G. et al. Pathogenic Mutations in the Kinesin-3 Motor KIF1A Diminish Force Generation and Movement Through Allosteric Mechanisms.Journal of Cell Biology (Arne Gennerich, Albert Einstein College of Medicine) 2021
Kraxner, J. et al. Post-Translational Modifications Soften Vimentin Intermediate Filaments. Nanoscale (Sarah Köster, University of Göttingen) 2020
Mei, L. et al. Molecular mechanism for direct actin force-sensing by α-catenin. eLife (Shixin Liu & Gregory M. Alushin, Rockefeller University) 2020
Schepers, A. et al. Tuning intermediate filament mechanics by indirect and direct charge variations. Nanoscale (Sarah Köster, University of Göttingen) 2020
Sorkin, R. et al.Synaptotagmin-1 and Doc2b Exhibit Distinct Membrane-Remodeling Mechanisms. Biophysical Journal (Gijs Wuite, VU University Amsterdam & Wouter Roos, Rijksuniversiteit Groningen) 2020
Zhang, Q. et al. The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation.Science Advances (Bo Sun, ShanghaiTech) 2019
Gutierrez-Escribano, et al. A conserved ATP- and Scc2/4-dependent activity for cohesin in tethering DNA molecules. Science Advances (David Rueda, & Luis Aragon, Imperial College London) 2019
Lorenz, C. et al.Lateral Subunit Coupling Determines Intermediate Filament Mechanics. Physical Review Letters (Sarah Köster, University of Göttingen) 2019
Forsting, J. et al.Vimentin Intermediate Filaments Undergo Irreversible Conformational Changes during Cyclic Loading. Nano Letters (Sarah Köster, University of Göttingen) 2019
Block, J.et al. Viscoelastic properties of vimentin originate from nonequilibrium conformational changes. Science Advances (Sarah Köster, University of Göttingen) 2018
Block, J. et al. Nonlinear Loading-Rate-Dependent Force Response of Individual Vimentin Intermediate Filaments to Applied Strain. Physical Review Letters (Sarah Köster, University of Göttingen) 2017

Zhao, Y. et al. Phase-separated ParB enforces diverse DNA compaction modes and stabilizes the parS-centered partition complex. Nucleic Acids Research 2024
Alshareedah, I. et al. Sequence-specific interactions determine viscoelasticity and ageing dynamics of protein condensates. Nature Physics 2024
Alshareedah, I. et al., Determinants of viscoelasticity and flow activation energy in biomolecular condensates. Science Advances 2024
Kota D. et al., Adenosine Triphosphate Mediates Phase Separation of Disordered Basic Proteins by Bridging Intermolecular Interaction Networks. JACS 2024
Wadsworth, G.M. et al., RNAs undergo phase transitions with lower critical solution temperatures. Nature Chemistry, (Priya Banerjee, University at Buffalo) 2023
Gui, T. et al. Targeted perturbation of signaling-driven condensates. Molecular Cell (Jurian Schuijers, UMC Utrecht)
Wadsworth, G. et al. RNAs undergo phase transitions with lower critical solution temperatures. Nature Chemistry (Priya Banerjee, University at Buffalo) 2023
Galvanetto, N. et al. Extreme dynamics in a biomolecular condensate. Nature (Ben Schuler, University of Zurich) 2023
Teng, Wang.et al. Bloom syndrome helicase compresses single-stranded DNA into phase-separated condensates. Angewandte Chemie International Edition (Bo Sun, ShanghaiTech) 2022
Kota, D, et al. Macromolecular Regulation of the Material Properties of Biomolecular Condensates. The Journal of Physical Chemistry Letters (Huan-Xiang Zhou, University of Illinois Chicago) 2022
Manjia Li. et al, Controlling synthetic membraneless organelles by a red-light-dependent singlet oxygen-generating protein. Nature Communication (Jingqing Huang, HKUST) 2022
Joris Van Lindt et al., F/YGG-motif is an intrinsically disordered nucleic-acid binding motif. RNA biology (Leuven) 2022
Ghosh, A. et al. Shear relaxation governs fusion dynamics of biomolecular condensates. Nature Communications, (Huan-Xiang Zhou, University of Illinois Chicago) 2021
Alshareedah, I. et al. Programmable viscoelasticity in protein-RNA condensates with disordered sticker-spacer polypeptides. Nature Communications (Priya Banerjee, University at Buffalo) 2021
Lin, SN. et al. Direct visualization of the effect of DNA structure and ionic conditions on HU–DNA interactions . Scientific Reports (Gijs Wuite, VU University Amsterdam) 2021
Newton, M. D. et al. A Minimal Load-and-Lock Ru Luminescent DNA Probe. Angew. Chem. Int. Ed. (David Rueda, Imperial College London) 2021
Rhine, K. et al. Single-molecule and ensemble methods to probe RNP nucleation and condensate properties. Methods (Sua Myong, Johns Hopkins University) 2021
Kaur, T. et al. Sequence-encoded and Composition-dependent Protein-RNA Interactions Control Multiphasic Condensate Morphologies. Nature Communications (Priya Banerjee, University at Buffalo) 2021
Alshareedah, I. et al. Quantifying Viscosity and Surface Tension of Multi-Component Protein-Nucleic Acid Condensates. Biophysical Journal (Priya Banerjee, University at Buffalo) 2021
Jawerth, L. et al. Protein condensates as aging Maxwell fluids. Science (Tony Hyman, MPI-CBG) 2020
Ghosh, A. and Zhou, H.-X. Determinants for Fusion Speed of Biomolecular Droplets. The Journal of Physical Chemistry B (Huan-Xiang Zhou, University of Illinois Chicago) 2020
Alshareedah, I. et al. Phase transition of RNA−protein complexes into ordered hollow condensates. PNAS (Priya Banerjee, University at Buffalo) 2020
Alshareedah, I. et al. Interplay between Short-Range Attraction and Long-Range Repulsion Controls Reentrant Liquid Condensation of Ribonucleoprotein–RNA Complexes. Journal of the American Chemical Society (Priya Banerjee, University at Buffalo) 2019
Gui, X., et al. Structural basis for reversible amyloids of hnRNPA1 elucidates their role in stress granule assembly. Nature Communications (Bo Sun, ShanghaiTech & Cong Liu, CAS-SIOC) 2019
Kaur, T. et al. Molecular Crowding Tunes Material States of Ribonucleoprotein Condensates. Biomolecules (Priya Banerjee, University at Buffalo) 2019
Jawerth, L. et al. Salt-Dependent Rheology and Surface Tension of Protein Condensates Using Optical Traps. Physical Review Letters (Tony Hyman, MPI-CBG) 2018

Winer, B. Y. et al. Plasma membrane abundance dictates phagocytic capacity and functional cross-talk in myeloid cells. Sci. Immunol. 2024
Kural, M. H. et al. Mechano-inhibition of endocytosis sensitizes cancer cells to Fas-induced Apoptosis. Cell Death Dis 2024
Vaknin, A. et al. Ebola Virus Glycoprotein Strongly Binds to Membranes in the Absence of Receptor Engagement. ACS Infect. Dis. 2024
Goren, S. et al. Micro-tensile rheology of fibrous gels quantifies strain-dependent anisotropy. Acta Biomaterialia 2024
Dharan, R. et al. Extracellular domain 2 of TSPAN4 governs its functions. Biophysical Reports 2024
Zhang, D. et al. Rethinking the relationships between gel like structure and sludge dewaterability based on a binary gel like structure model: Implications for the online sensing of dewaterability. Water Research 2024
Evers, T.M.J. et al. Single-cell analysis of innate immune cell mechanics: an application to cancer immunology. Material Advances 2024
Jo, M.H. et al. Determination of single-molecule loading rate during mechanotransduction in cell adhesion Science 2024
Evers, T.M.J. et al. Mechanics of serotonin-producing human entero-endocrine cells. Mechanobiology in Medicine2024
Liu, D. et al. Dynamic encounters with red blood cells trigger splenic marginal zone B cell retention and function, Nature Immunology 2023
De Belly, H. et al. Cell protrusions and contractions generate long-range membrane tension propagation. Cell (Carlos Bustamante, UC Berkeley) 2023
Cheng Lyu et al., Advanced glycation end-products as mediators of the aberrant crosslinking of extracellular matrix in scarred liver tissue. Nature Biomedical Engineering (Yanan Du, Tsinghua university) 2023
Zhorabek F. et al., Construction of multiphasic membraneless organelles towards spontaneous spatial segregation and directional flow of biochemical reactions. Chemical Science (Jinqing Huang, Hong Kong UST) 2023
Stadler R. et al., A circular zone of attachment to the extracellular matrix provides directionality to the motility of Toxoplasma gondii in 3D. Elife (Gary E. Ward, Vermont University) 2022
Goren S. et al., Probing Local Force Propagation in Tensed Fibrous Gels, Small (Raya Sorkin, Tel Aviv) 2022
Quikun Y. et al., Directed assembly of genetically engineered eukaryotic cells into living functional materials via ultrahigh-affinity protein interactions. Sci. Advances (Jinqing Huang, Hong Kong UST) 2022
Dharan R. et al., Transmembrane proteins tetraspanin 4 and CD9 sense membrane curvature. PNAS (Raya Sorkin, Tel Aviv) 2022
Siahaan, V. et al. Microtubule Lattice Spacing Governs Cohesive Envelope Formation of Tau Family Proteins. Nature Chemical Biology (Zdenek Lansky, BIOCEV) 2022
Mulla, Y. et al. Weak catch bonds make strong networks. Nature Materials (Sander Tans, AMOLF & Gijsje Koenderink, TU Delft) 2022
Evers T. et al., Single-Cell Mechanical Characterization of Human Macrophages. Advanced Nanobiomed Research (Alireza, Leiden University) 2022
Sheikhhassani, V.  et al., Single cell force spectroscopy of erythrocytes at physiological and febrile temperatures reveals mechano-modulatory effects of atorvastatin. Soft matter, (Mashaghi, Leiden) 2022
Evers, T. M. J. et al. Single-cell analysis reveals chemokine-mediated differential regulation of monocyte mechanics. iScience, (Alireza Mashaghi , Leiden University) 2022
Vasse, G. F. et al. Single Cell Reactomics: Real‐Time Single‐Cell Activation Kinetics of Optically Trapped Macrophages. small methods, (Wouter Roos, Rijksuniversiteit Groningen) 2021

Liu, Z. et al., A Biophysics Toolbox for Reliable Data Acquisition and Processing in Integrated Force–Confocal Fluorescence Microscopy. ACS Photonics 2024
Xu, H. et al., 3D nanofabricated soft microrobots with super-compliant picoforce springs as onboard sensors and actuators, Nature Nanotechnology 2024
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Our solution

The C-Trap^® Optical Tweezers – Fluorescence & Label-free Microscopy is the world’s first instrument that allows simultaneous manipulation and visualization of single-molecule interactions in real time. It combines high resolution optical tweezers, fluorescence and label-free microscopy and an advanced microfluidics system in a truly integrated and correlated solution.

The C-Trap offers you a fast workflow to seamlessly catch and manipulate single molecules. The instrument measures their structural changes or interactions while you visualize them in teal time with high spatial and temporal resolution, ultimately offering you a complete and detailed picture of biomolecular properties and interactions