Skip to main content

Exonucleolysis and Replication by T7 DNA Polymerase Studied using Optical Tweezers and AFS™


The paper “Switching between Exonucleolysis and Replication by T7 DNA Polymerase Ensures High Fidelity” has recently been published in the Biophysical Journal using LUMICKS’ AFS™ technology and optical tweezers. We would like to congratulate all the authors!

In the paper by Hoekstra et al., optical tweezers and AFS™ were used to study the kinetic interplay between replication and proofreading by probing how the effect of DNA-duplex stability affects the bacteriophage T7 DNA polymerase replication process. They found that DNA polymerase dissociation-mediated DNA transfer is the allocated pathway for error correction and that this is a stochastic process prone to false positives to avoid the contrary, rather than a precise control mechanism. This “…ensures the high-fidelity replication of the bacteriophage T7 genome”.

The techniques used in this paper are made commercially available by LUMICKS. Read more about the C-Trap® optical tweezers and AFS™.

Close Menu

For pricing, reach out to your application scientist or account manager

Open Email

Fill in our contact form, we will reach out to you!

Please include ‘Lakeview data analysis price inquiry’ in the Message box

Contact Us

Join our newsletter

Get exclusive news on the latest publications, product developments, events and breakthrough science.

By submitting the form you agree to LUMICKS' privacy policy. You can revoke your consent at any time.

Download our webinar recording:


Download our Cell Therapy (CAR-T, TCR, NK) applications deck