Revealing biomolecular insights never before available
Molecular mechanisms are at the heart of understanding biology
All biological processes ultimately take place at the molecular level, all diseases arise at this level, and practically all drugs act at this level. However, many life science and tools that are used today to approximate and extract molecular function such as structural biology, bulk functional assays, cell imaging, and localization assays, give either detailed structural or functional information - but rarely both.
Unable to observe molecular processes in real time, they often fail to reveal crucial mechanistic details of the molecular factors at play. This lack of direct observation of the underlying dynamic processes often results in ambiguous data, not suited to deliver clear insights.
Unable to observe molecular processes in real time, they often fail to reveal crucial mechanistic details of the molecular factors at play. This lack of direct observation of the underlying dynamic processes often results in ambiguous data, not suited to deliver clear insights.
Dynamic Single-Molecule, the missing link
What if you could measure molecular properties and interactions while simultaneously recording and showing you the processes in real-time? A technology that can reveal the crucial and dynamic interactions taking place at the molecular level and gives you direct proof of the mechanisms involved. A tool that can provide an understanding of the root of disease development at the molecular level and accelerate therapeutic breakthroughs.
Dynamic Single-Molecule combines live visualization, manipulation, and force measurement at the smallest molecular scale, leads to single-molecule imaging and base-pair resolution measurements of the interactions between biomolecules. Taken together, this provides – for the first time – the crucial dynamic and functional mechanistic information that is complementary to molecular structure, bulk functional assays, and cell imaging.
Dynamic Single-Molecule combines live visualization, manipulation, and force measurement at the smallest molecular scale, leads to single-molecule imaging and base-pair resolution measurements of the interactions between biomolecules. Taken together, this provides – for the first time – the crucial dynamic and functional mechanistic information that is complementary to molecular structure, bulk functional assays, and cell imaging.
Gain unprecedented insights into the complex mechanistic details of interactions
Correlate structure and function like no other technology can do
Get started up in no time through the easy to use workflow and solutions
Workflow
The Dynamic Single-Molecule workflow
Let’s take you through the various steps involved in an experiment
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Applications
Explore your research application
Explore what Dynamic Single-Molecule can mean for your field of interest
DNA-Binding Proteins
DNA Replication
Study and visualize DNA replication mechanisms at the nanoscale
Use Dynamic Single-Molecule to obtain the full understanding of replication mechanisms
Available case studies:
The replication machinery: Identify key players and their diverse roles
Case study
Real-time insights into origin recognition and replisome formation
Case study
Replication in context: Uncover mechanisms ensuring replication fidelity and genome stability
Case study
DNA Transcription
Study and visualize DNA transcription mechanisms at the nanoscale
Use Dynamic Single-Molecule to obtain the full understanding of transcription mechanisms
Available case studies:
Real-time observation of DNA exonuclease dynamics at base-pair level
Case study
DNA Repair
Study and visualize DNA repair processes at the nanoscale
Use Dynamic Single-Molecule to obtain the full understanding of repair mechanisms
Available case studies:
Characterize the (dis-)assembly kinetics of repair complexes based on single-molecule real-time data
Case study
Directly observing molecular search and repair mechanisms delivers unexpected insights
Case study
Pulling on individual molecules reveals how biomolecular condensation physically prevents DNA end disjunction
Case study
DNA Organization
Mechanisms and roles of chromatin organization – decipher the epigenetic code
Available case studies:
Quantify SMC activity, conformation and interactions at the molecular level
Case study
Follow chromatin remodeler activity in real-time
Case study
Use the force: Quantify nucleosome stability and cross-linking
Case study
DNA/RNA Structure
Reveal the structural dynamics of RNA & DNA in real time
Use Dynamic Single-Molecule to obtain the full understanding of DNA/RNA structure
Available case studies:
Uncover structural dynamics in telomeric RNA for cancer research
Case study
Investigate RNA misfolding in neurodegenerative disorders
Case study
Reveal protein-RNA interactions critical for viral replication
Case study
DNA Editing
Study and visualize DNA editing mechanisms on the nanoscale
Use Dynamic Single-Molecule to obtain the full understanding of editing mechanisms
Available case studies:
Understand off-target activities of Cas for safer gene editing
Case study
Real-time insights into gene editing mechanisms
Case study
Mechanobiology
Uncover mechanical principles of cellular function at the molecular level
Available case studies:
Kinetochore-mediated chromosome segregation
Case study
Cellular tension propagation revisited
Case study
Balancing pharmacokinetic benefits with cell avidity optimization for cell engager success
Case study
Phase Separation
From fundamental mechanism to biological function – explore biomolecular condensates across scales
Available case studies:
Variations in low-complexity domains and how they influence protein droplet solidification
Case study
The effect of molecular crowders on droplet fusion
Case study
The effect of RNA/RNP interactions on the fusion of protein droplets
Case study
Cytoskeletal Structure and Transport
Study cytoskeletal motors in real-time at the nanoscale
Available case studies:
Investigation of motor protein stepping mechanics along cytoskeletal filaments
Case study
Stepping of filaments and motors at the surface
Case study
Force-extension, manipulation, and visualization of polymers and protein filaments
Case study
Protein Folding
Study protein folding and conformational dynamics at the nanoscale
Available case studies:
Study of multi-domain protein folding & conformational dynamics
Case study
Solutions
C-Trap
Biomolecular interactions re-imagined
The C-Trap® provides the world’s first dynamic single-molecule microscope to allow simultaneous manipulation and visualization of single-molecule interactions in real time.
Technical note:
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Text Link
Migrasome formation is initiated preferentially in tubular junctions by membrane tension
Zucker, B. et al.
2025
Biophysical Journal
https://doi.org/10.1016/j.bpj.2024.12.029
Publication
Text Link
E. coli RecB Nuclease Domain Regulates RecBCD Helicase Activity but not Single Stranded DNA Translocase Activity
Fazio, N. et al.
2024
Journal of Molecular Biology
https://doi.org/10.1016/j.jmb.2023.168381
Publication
Text Link
Rapid Long-distance Migration of RPA on Single Stranded DNA Occurs Through Intersegmental Transfer Utilizing Multivalent Interactions
Pangeni, S. et al.
2024
Journal of Molecular Biology
https://www.sciencedirect.com/science/article/pii/S0022283624000639
Publication
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New Insights in Chromosome Organization: Single Molecule Analysis of Eukaryotic SMC Complexes
Webinar
March 31, 2025
01-01-20
Structural Maintenance of Chromosome (SMC) complexes, cohesin, condensin and Smc5/6, play a fundamental role in genome organization, facilitating chromosome compaction, segregation, and DNA repair. Despite their essential functions, the mechanisms by which the complexes interact with different DNA substrates and influence topological transitions remain not fully understood. Using the LUMICKS C-Trap, we have employed single-molecule approaches to analyze the behavior of purified SMC complexes, focusing on yeast cohesin and human SMC5/6, on different DNA substrates, including double-stranded (dsDNA) and single-stranded DNA (ssDNA). Additionally, we have used a quadrupole optical trap to bridging by SMC complexes and their effect on DNA decatenation by Topoisomerase IIα (Top2A). These findings provide new insights into the fundamental properties and requirements of cohesin and Smc5/6’s interaction with DNA substrates, as well as their ability to bridge two independent DNAs.
Text Link
Linking Mechanical Stability with in vivo Recombination: Single-molecule Research Reveals Bacterial Antibiotic Resistance
Scientific update
January 4, 2025
01-01-20
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Golden Gate meets C-Trap: A powerful combination for unprecedented molecular insights
Application note
December 18, 2024
01-01-20
Precisely manipulating genetic material at the single molecule level is gaining importance across life sciences – and so do the tools that allow researchers to do exactly that. The C-Trap system combines single molecule fluorescence microscopy with optical tweezers to manipulate DNA, allowing researchers to directly observe and track molecular events as they occur. Designing and creating specific DNA constructs is crucial for maximizing the potential of single molecule studies. In this application note we introduce the powerful combination of cutting edge biochemistry and single-molecule visualization methods to increase throughput and maximize the results gained from each individual measurement.
C-Trap Product Brochure
Brochure
February 28, 2025
01-01-20
AACR 2025
Conference
April 18, 2025
01-01-20
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We empower the academic and pharmaceutical communities with cutting-edge technologies to deeply understand the mechanisms of life and disease, driving the discovery and development of life-saving therapies
All products and technologies are for Research Use Only (RUO) except when specifically noted