Observe your protein on DNA in real-time to directly connect its molecular mechanisms to its clinical impact.
Find out how you can gain these unique insights
Mechanisms are by their nature dynamic. This is combined with the challenge that not all mechanisms act the same way all of the time. How can you get the insight you need to fully understand your protein DNA binding mechanism? Read our application note
Important highlights:
– Direct evidence of the DNA/protein binding site
– New insights into clinical impact uncovered
– How unambiguous, direct evidence was obtained
Learn how experts think about dynamic single-molecule:
Prof. David Rueda, corresponding author of the original study
Understanding how DNA-binding proteins interact with DNA is key
For example, by looking at the interactions of POLQ with DNA, a recent study suggests that the inhibition of its DNA-repair capabilities could play a synergetic role with the inhibition of another DNA repair protein, PARP. Indeed, targeting both proteins in novel cancer therapies could result in the efficient killing of cancer cells.
Adapted from Belan et al. Molecular Cell (2022)
Current methods often struggle to reveal the dynamic molecular mechanism of individual proteins on DNA
The protein’s molecular function can be inferred through methods that examine its detailed static structure or average behavior. However, these methods are often unable to reveal some of the crucial mechanistic details that are only accessible when studied:
Looking dynamically
DNA-protein interactions are inherently dynamic. Not only are the beginning and end states important, but all the intermediate states too. These can only be captured by a dynamic movie looking at every step of the process
In real-time
Watching a dynamic movie of DNA-protein interactions in real-time gives the ability to have a direct influence over the movie, as it unfolds. This enables unique conditions and functions to be investigated
At the single-molecule level
When looking at a large number of proteins, the average protein function can hide sub-population or individual behaviors. Behaviors that are functionally key and that are only visible when looking at a single protein
With great experimental control
Having control over one specific piece of DNA, which proteins it is interacting with and in which condition it is really unlocks unique ways to probe and understand protein function
What if a method exists that fulfills all these requirements?
A dynamic single-molecule method for direct, indisputable proof of the detailed molecular mechanisms
Directly visualize the location and dynamics of individual biomolecules
Control the stepwise assembly of the biological complex
Modulate molecular conformations while observing changes as they happen
The C-Trap® is the world’s first
dynamic single-molecule instrument
Designed to capture detailed DNA-binding protein interactions in real-time,
effortlessly, leading you in no time to highly impactful discoveries.
We are trusted worldwide by key opinion leaders that are already using the C-Trap to conduct highly impactful science
Senior vice-president at Artios Pharma Ltd and senior group leader at the Francis Crick Institute in London
Group leader at Vrije Universiteit Amsterdam
Distinguished Professor of Microbiology and Molecular Genetics at the University of California at Davis.
Top C-Trap publications you can’t miss
Greenhough L. A. et al, Structure and mechanism of action of the RAD51BCD-XRCC2 tumour suppressor complex, Nature, 2023 (Simon Boulton & Steve West, The Francis Crick Institute, London, UK)
Anand, R. et al. HELQ is a dual-function DSB repair enzyme modulated by RPA and RAD51. Nature, 2021. (Simon Boulton, The Francis Crick Institute, London, UK)
Wasserman, M. et al. Replication Fork Activation Is Enabled by a Single-Stranded DNA Gate in CMG Helicase. Cell 2019. (Shixin Liu & Michael O’Donnel, Rockefeller University, New York, US)
Ling Wang et. al., Head-on and co-directional RNA polymerase collisions orchestrate bidirectional transcription termination. Molecular Cell 2023. (Shixin Liu, Rockefeller University, New York, US)
Publish more impactful science, faster
Publish quickly and regularly in high impact journals.
Our customer are getting initial results within one week of their training, leading to publishing their first impactful studies in under 2 years.
Did you know, on average our C-Trap users are now publishing every 10 months? Not just that, but in journals with an average impact factor of 13!
Take the next step into uncovering unique molecular mechanisms
