Optical Tweezers – Fluorescence Microscopy

The C-Trap™ is the world’s first instrument that combines optical tweezers, confocal microscopy or STED nanoscopy and an advanced microfluidics system in a truly integrated and correlated way.

Watch our short introduction video!


Optical Tweezers-Fluorescence Microscopy

The C-Trap™ is the world’s first instrument that combines optical tweezers, confocal microscopy or STED nanoscopy (SuperC-Trap™) and an advanced microfluidics system in a truly integrated and correlated way. The (Super)C-Trap™ is capable of live, simultaneous and correlative visualization and manipulation of molecular interactions such as DNA-protein interactions with sub-picoNewton force resolution and sub-nanometer position resolution. Explore the applications below to see what this technology can mean for you.

Applications

Correlative Tweezers – Fluorescence Microscopy (CTFM) reveals unprecedented detailed information on a wide range of molecular mechanisms, including, but not limited to: DNA repair, DNA organization, chromatin organization, DNA replication, DNA transcription, DNA-DNA interactions, DNA/RNA structural dynamics, DNA hairpin dynamics, biomolecular motors, protein folding, protein unfolding, ribosome translation, intermediate filaments, cell membrane interactions and cell motility mechanisms.

Browse our list of detailed C-Trap™ applications below.

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Measure Multi-Domain Protein Unfolding

Single-Molecule Visualization of DNA Organization

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Force Extension, Manipulation & Visualization of DNA Repair

Single-Molecule Visualization of DNA Repair

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Measure Activity & States of Transcription with Force & Fluorescence

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Measure DNA Replication Activity & States with Force & Fluorescence

Single-Molecule Visualization of DNA Replication

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Force Extension, Manipulation & Visualization of Polymers & Protein Filaments

Single-Molecule Visualization of Transcription

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Measure Conformational Changes of DNA Organization

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Force Extension, Manipulation & Visualization of DNA Organization

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Small Molecule Influence on Enzymatic Activity

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Dynamics of Membrane Proteins and Droplet Fusion

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Super-Resolution Single-Molecule Visualisation using STED Nanoscopy

DNA-RNA Mechanics

Force Extension, Manipulation & Visualization of DNA/RNA structural dynamics

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Single-Molecule Visualization of Cytoskeletal Motors

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Single restriction proteins bound to a DNA molecule. Optically trapped between two microspheres and visualized using single colour confocal microscopy, both done simultaneously in the C-Trap™ instrument.

Features

The C-Trap™ can be adapted to your needs, allowing for up to four individually controllable traps, single to multi-color confocal fluorescence microscopy or an upgrade to STED super-resolution.

Optical Tweezers

Multiple continuous optical traps, dual (2) or quadruple (4) configurations
Unparalleled range of trap stiffness and low force noise
Absolute 3D trap positioning

Confocal Fluorescence

Multicolor (up to 3 colors) confocal scanning fluorescence microscopy
Single-photon sensitivity
Upgradable to STED super resolution

u-Flux™ Microfluidics

Ultra-stable passive pressure driven microfluidics
Monolithic laminar flow cell with automated valve switching
Truly correlative force-fluorescence data acquisition

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Intuitive Software & Workflow

The C-Trap™ dual-screen software interface allows you to automate your single-molecule assay in an intuitive matter, for a high throughput and repeating experiments. Controls such as optical trap positions, stage positions, microfluidics and data recording are executed manually through simple clicks with the joystick or automatically through a simple command scripting feature.

 

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