Use avidity to identify the best NK cell with optimal response
In addition to autologous cellular therapy, allogeneic strategies may facilitate an off-the-shelf solution, including NK cells as a prime example.
In this assay, researchers from Glycostem Therapeutics B.V. compared cell avidity data with cell killing by evaluating NK functionalities from two different donors (donor 1 and donor 2).
NK cells from donor 2 induced 3-fold higher cytotoxicity compared with NK cells from donor 1 (74% and 24%, respectively). NK cells from donor 2 required higher forces than those from donor 1 to detach from target cells (Figure 1).
The data suggest that cell avidity is a reliable predictor of NK cell function and that cell avidity analyses can be applied to different types of immune cells.
1 Left: Cytotoxic effect of NK cells from donor 1 and donor 2 on the same population of target cells. Right: Avidity curve showing the proportion of target-bound NK cells upon application of a force ramp. (rForce indicates relative force).