Use avidity to identify the best NK cell with optimal response
In addition to autologous cellular therapy, allogeneic strategies may facilitate an off-the-shelf solution, including NK cells as a prime example.
In this assay, researchers from Glycostem Therapeutics B.V. compared cell avidity data with cell killing by evaluating NK functionalities from two different donors (donor 1 and donor 2).
NK cells from donor 2 induced 3-fold higher cytotoxicity compared with NK cells from donor 1 (74% and 24%, respectively). NK cells from donor 2 required higher forces than those from donor 1 to detach from target cells (Figure 1).
The data suggest that cell avidity is a reliable predictor of NK cell function and that cell avidity analyses can be applied to different types of immune cells.
1 Left: Cytotoxic effect of NK cells from donor 1 and donor 2 on the same population of target cells. Right: Avidity curve showing the proportion of target-bound NK cells upon application of a force ramp. (rForce indicates relative force).
The z-Movi® is a unique instrument that measures the avidity between immune cells and their targets, enabling you to identify the most potent immunotherapeutic effector cells. This new technology provides you with predictive, reproducible, and fast high-throughput results at a single-cell resolution without compromising cell viability. All within a compact little box that easily fits inside the flow hood for sterile and safe sample handling
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