Use avidity to identify the best NK cell with optimal response
In addition to autologous cellular therapy, allogeneic strategies may facilitate an off-the-shelf solution, including NK cells as a prime example.
In this assay, researchers from Glycostem Therapeutics B.V. compared cell avidity data with cell killing by evaluating NK functionalities from two different donors (donor 1 and donor 2).
NK cells from donor 2 induced 3-fold higher cytotoxicity compared with NK cells from donor 1 (74% and 24%, respectively). NK cells from donor 2 required higher forces than those from donor 1 to detach from target cells (Figure 1).
The data suggest that cell avidity is a reliable predictor of NK cell function and that cell avidity analyses can be applied to different types of immune cells.
1 Left: Cytotoxic effect of NK cells from donor 1 and donor 2 on the same population of target cells. Right: Avidity curve showing the proportion of target-bound NK cells upon application of a force ramp. (rForce indicates relative force).
The z-Movi® is a unique instrument that measures the avidity between immune cells and their targets, enabling you to identify the most potent immunotherapeutic effector cells. This new technology provides you with predictive, reproducible, and fast high-throughput results at a single-cell resolution without compromising cell viability. All within a compact little box that easily fits inside the flow hood for sterile and safe sample handling
Analytical cookies are used to understand how visitors interact with the website. These cookies help provide information on metrics the number of visitors, bounce rate, traffic source, etc.
The _ga cookie, installed by Google Analytics, calculates visitor, session and campaign data and also keeps track of site usage for the site's analytics report. The cookie stores information anonymously and assigns a randomly generated number to recognize unique visitors.
Set by Google to distinguish users.
Provided by Google Tag Manager to experiment advertisement efficiency of websites using their services.
Installed by Google Analytics, _gid cookie stores information on how visitors use a website, while also creating an analytics report of the website's performance. Some of the data that are collected include the number of visitors, their source, and the pages they visit anonymously.
Clarity is a user behavior analytics tool that helps you understand how users are interacting with our website through session replays and heatmaps.
Google Analytics Universal
A variation of the _gat cookie set by Google Analytics and Google Tag Manager to allow website owners to track visitor behaviour and measure site performance. The pattern element in the name contains the unique identity number of the account or website it relates to.
The pardot cookie is set while the visitor is logged in as a Pardot user. The cookie indicates an active session and is not used for tracking.