Optical tweezers combined with STED reveals protein dynamics on densely covered DNA
Today’s quantitative analysis of DNA-protein interactions at the single-molecule level requires the capability of measuring under biologically relevant conditions with high temporal resolution. This problem can especially be observed when in vitro experiments are linked with the in vivo situation in which proteins interact highly dynamic with DNA at high protein concentrations. These experiments can be conducted when fast DNA scanning is combined with stimulated emission depletion (STED) microscopy. In this application note we will discuss how LUMICKS’ C-Trap® Dymo enables the visualization of individual DNAbinding proteins on densely covered DNA.
1. The use of STED enables the visualization of individual DNA binding proteins on densely covered DNA in high resolution, therefore enhancing the localization accuracy of proteins on DNA, which cannot be observed with the diffraction-limited.
2. Resolution of confocal microscopy.
3. LUMICKS’ C-Trap® Dymo, the optical tweezers combined with STED microscopy, was used for real-time observation of dynamics of TFAM on lambda-DNA at high concentration.