Learn how the use of nuclear extracts for single-molecule studies of DNA-protein interactions opens up new possibilities for molecular biology research.
Adrià Ferragut17 December 2024
CRISPR Cas9 is a gene editing tool that has increased in popularity due to its simplicity to use. It allows researchers to seamlessly edit DNA sequences by combining a sequence identifying guide RNA (gRNA) with the Cas9 endonuclease enzyme. However, its applicability as a gene-editing and therapeutic tool is impeded…
Adrià Ferragut27 October 2023
DNA repair, the collection of highly regulated mechanisms by which a cell identifies and repairs DNA damage, remains one of the most essential processes of human life. Impaired DNA repair systems may lead to malignant mutations that jeopardize cellular well-being. To study DNA repair, single-molecule studies have proven to greatly…
Adrià Ferragut27 October 2023
Today’s quantitative analysis of DNA-protein interactions at the single-molecule level requires the capability of measuring under biologically relevant conditions with high temporal resolution. This problem can especially be observed when in vitro experiments are linked with the in vivo situation in which proteins interact highly dynamic with DNA at high…
Adrià Ferragut27 October 2023
In this application note, we highlight multiple experiments conducted at Banerjee Lab at the University at Buffalo, and Hyman Lab at the Max Planck Institute of Molecular Cell Biology and Genetics on phase separation using the C-Trap dynamic single molecule technology. We present the two approaches the studies used to…
Adrià Ferragut27 October 2023
DNA repair is a highly complex and dynamic process that involves the interplay of numerous different proteins and components. The helicase HELQ is known to play a role in double-stranded breaks (DSBs) repair, but its molecular mechanisms remain unknown. A study by the research group led by Simon Boulton presented…
Adrià Ferragut27 October 2023
In this application note, we describe the power of cell avidity in the search for improved CAR T cell therapies. We report on two publications from the lab of Dr. Marcela V. Maus (Harvard Medical School) that illustrate how cell avidity measurement with the z-Movi Cell Avidity Analyzer can accelerate…
getyos14 September 2023
In this research highlight, we present cell avidity results obtained with the z-Movi® Cell Avidity Analyzer in collaboration with the team of Dr. Maria Themeli (VUMC Cancer Center Amsterdam) working on BCMA + CD38 dual-targeting CAR T therapy against multiple myeloma. Key learning points: Cell avidity measurements with the z-Movi…
getyos14 September 2023
In this application note, we present cell avidity obtained with the z-Movi® Cell Avidity Analyzer and in vitro experiments performed in collaboration with the team of Dr. Nathalie Rufer (University of Lausanne). Here you will learn: 1. In contrast to affinity measured by SPR, cell avidity measurements directly correlate with…
getyos14 September 2023
