Acinetodin and Klebsidin Lasso Peptides Studied with AFS™ Technology

The first paper from one of our clients using AFS technology has been published in the journal ACS Chemical Biology led by prof. Mikhail Khodorkovskii. Congratulations to all the authors!

In the paper named “Acinetodin and Klebsidin, RNA Polymerase Targeting Lasso Peptides Produced by Human Isolates of Acinetobacter gyllenbergii and Klebsiella pneumoniae” Prof. Khodorkovskii and his group study the bioinformatic prediction and structural validation of two new lasso peptides encoded by several human-associated species of Klebsiella and Acinetobacter: acinetodin and klebsidin. Using the AFS™, they performed kinetic analysis of transcription elongation by E.coli RNA polymerase, showing that these lasso proteins bind to a common site on the Escherichia coli genome, inhibiting transcription as well as transcription elongation by the E. coli RNA polymerase.