Step into the

unresolved

with the

Surface Assay Toolkit

3 cutting-edge imaging techniques seamlessly integrated with optical tweezers to deliver ultra-stable surface assays at the single molecule level:

IRM

Out now!

TBA

BPS 2019

TBA

BPS 2019

We’re excited to introduce the first of three new imaging techniques: Label-free imaging using Interference Reflection Microscopy (IRM), with the other two following up soon on March 6 at the Biophysical Society Annual Meeting.

Go Label-free with IRM

Advanced fluorescence microscopy, though reliable, often requires laborious biochemical preparation and can negatively affect experiments due to photobleaching effects or by altering the biological function of the specimens being studied. For cytoskeletal assays, these disadvantages can come in the way of making breakthrough discoveries.

Interference Reflection Microscopy (IRM) is a technique known for providing high resolution, quantifiable images of filaments on a glass surface. IRM allows seamless integration with optical tweezers and offers the highest contrast and temporal resolution.

The movie shows a typical field of view showing microtubules on the surface. Captured using the surface assay toolkit: IRM integrated to the C-Trap™ optical tweezers. Sections of the microtubules are not rigidly bound to the glass and can freely fluctuate in solution.  Distinct patterns composed of alternating light and dark stripes are created by the IRM imaging method. This provides insights into the 3D orientation of the microtubules.

Investigate the dynamic function, transport and structure of the cytoskeleton

compatible with both the C-Trap™ and m-Trap™ optical tweezers, the Surface Assay Toolkit enables you to investigate the dynamic function and structure of the cytoskeleton with the highest ease-of-use and performance. Reconstitute in vitro dynamic microtubule assays to visualize and measure intricate phenomena like protein binding, microtubule disassembly, severing and cargo transport. See the assays below to get a glimpse of the experimental possibilities.

Microtubule growth and catastrophe visualization at video rate

Microtubule–Microtubule sliding assay induced by microtubule-associated proteins (MAPs) performed with simultaneous visualization, force and distance measurements

Surface-tethered microtubule attached to an optically-trapped bead for the measurement of growth and shrinkage. In this assay, it is possible to apply along the direction of the filament force and measure precisely force and distance values with sub-pN and sub-nm resolution

Gliding assay to analyze microtubule-based motor dynamics

Mobility assay with a bead connected to a motor via a DNA linker and a surface attached substrate.

The highest force resolution and lowest drift at surface

Reliable hours-long measurements of the smallest mechanical changes with the new Active Stabilization Control and Precise Sample Control features

The highest
ease-of-use

You put the sample in, the software does the rest. With the new label-free imaging we have developed a whole new set of software features to make your experiments complete and enable the highest experimental throughput.

The most stable label-free imaging experiments

No photobleaching, no altering functionality, no label preparations, near physiological conditions. IRM allows you to visualize biological structures with the highest contrast and temporal resolution.

The shortest
time-to-result

The high throughput and ease-of-use of the system will give you publishable results straight away.

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