Surface Assay Toolkit
3 cutting-edge imaging techniques seamlessly integrated with optical tweezers to deliver ultra-stable surface assays at the single molecule level:
We’re excited to introduce the first of three new imaging techniques: Label-free imaging using Interference Reflection Microscopy (IRM), with the other two following up soon on March 6 at the Biophysical Society Annual Meeting.
Go Label-free with IRM
Advanced fluorescence microscopy, though reliable, often requires laborious biochemical preparation and can negatively affect experiments due to photobleaching effects or by altering the biological function of the specimens being studied. For cytoskeletal assays, these disadvantages can come in the way of making breakthrough discoveries.
Interference Reflection Microscopy (IRM) is a technique known for providing high resolution, quantifiable images of filaments on a glass surface. IRM allows seamless integration with optical tweezers and offers the highest contrast and temporal resolution.
The movie shows a typical field of view showing microtubules on the surface captured using the surface assay toolkit: IRM integrated to the C-Trap™ optical tweezers. Sections of the microtubules are not rigidly bound to the glass and can freely fluctuate in solution. Distinct patterns composed of alternating light and dark stripes are created by the IRM imaging method. This provides insights into the 3D orientation of the microtubules.
Investigate the dynamic function, transport and structure of the cytoskeleton
compatible with both the C-Trap™ and m-Trap™ optical tweezers, the Surface Assay Toolkit enables you to investigate the dynamic function and structure of the cytoskeleton with the highest ease-of-use and performance. Reconstitute in vitro dynamic microtubule assays to visualize and measure intricate phenomena like protein binding, microtubule disassembly, severing and cargo transport. See the assays below to get a glimpse of the experimental possibilities.
The highest force resolution and lowest drift at surface
Reliable hours-long measurements of the smallest mechanical changes with the new Active Stabilization Control and Precise Sample Control features
You put the sample in, the software does the rest. With the new label-free imaging we have developed a whole new set of software features to make your experiments complete and enable the highest experimental throughput.
The most stable label-free imaging experiments
No photobleaching, no altering functionality, no label preparations, near physiological conditions. IRM allows you to visualize biological structures with the highest contrast and temporal resolution.
The high throughput and ease-of-use of the system will give you publishable results straight away.