It all starts with the acoustics
At the heart of the z-Movi® technology, inside the microfluidic chip, you will find the piezo element that generates resonant acoustic waves. These ultrasound waves are the foundation of cell avidity analyses, as they can pull cells vertically toward the first acoustic node where the force is zero. You can easily control the force magnitude of the waves by regulating the voltage.
This seamless approach to separate interacting cells enables you to directly measure and record cell-specific avidities between populations of effector cells.
Effector cells will detach from the monolayer in a force-dependent manner and accumulate at the acoustic node. You can follow the detaching of the fluorescently labeled effector cells from the top view of the flow channel.
Figures a-f show the force-induced detachment of fluorescent effector cells establishing low avidity and high avidity interactions. Low avidity effector cells will rapidly release from the target cell monolayer (a-c, green trendline and column in the graphs below) and gather at the acoustic nodes (c), while high avidity effector cells require larger forces to rupture the binding strength (d-f, orange trendline and bar in the graphs below).
Rapid experiments with the optimized workflow
We have optimized the workflow of the z-Movi to facilitate the user experience and provide you with reliable, reproducible, and fast results. The z-Movi Chip is specialized to simplify cell culturing and maintain your samples in physiological conditions while you perform the experiments. Once you have performed one run of analyses, you can simply flush in a new batch of effector cells – up to 5 times!