DNA Mechanics Application Note

Summary

Single-molecule force spectroscopy (SMFS) tools are widely used to study structural transitions of DNA during overstretching. However, as many of these tools only provide global information, the exact mechanisms that occur during these transitions remain unclear. Combining SMFS with visualization of local information could resolve this problem. In this application note we will discuss how LUMICKS’ C-Trap™ technology combines high-resolution optical tweezers as a SMFS tool with confocal fluorescence microscopy to monitor the transition of doublestranded (ds) DNA to single-stranded (ss) DNA upon applying mechanical stress.

Download PDF

Comments are closed.

Take your research to the next level.

Get exclusive news on the latest products, single-molecule events and breakthrough science.

Newsletter pop up
By clicking the subscribe button you agree to LUMICKS’ privacy policy.