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Understanding how DNA-binding proteins interact with DNA is key
For example, by looking at the interactions of POLQ with DNA, a recent study suggests that the inhibition of its DNA-repair capabilities could play a synergetic role with the inhibition of another DNA repair protein, PARP. Indeed, targeting both proteins in novel cancer therapies could result in the efficient killing of cancer cells.
Current methods often struggles to reveal the dynamic molecular mechanism of individual proteins on DNA
The protein’s molecular function can be inferred through methods that examine its detailed static structure or average behavior. However, these methods are often unable to reveal some of the crucial mechanistic details that are only accessible when:
At the single-molecule level
With great experimental control
DNA-protein interactions are inherently dynamic. Not only are the beginning and end states important, but all the intermediate states too. These can only be captured by a dynamic movie looking at every step of the process
Watching a dynamic movie of DNA-protein interactions in real-time gives the ability to have direct influence over the movie, as it unfolds. This enables unique conditions and functions to be investigated
When looking at a large number of proteins, the average protein function can hide sub-population or individual behaviors. Behaviors that are functionally key and that are only visible when looking at a single protein
Having control over one specific piece of DNA, which proteins it is interacting with and in which condition it is really unlocks unique ways to probe and understand protein function
What if a method exists that fulfills all these requirements?
A dynamic single-molecule method for direct, indisputable proof of the detailed molecular mechanisms
Directly visualize the dynamics of individual proteins in real-time
Control and observe the stepwise assembly of the biological complex
Modulate the molecular system to probe different conditions
The C-Trap® is the world’s first dynamic single-molecule instrument
designed to capture detailed DNA-binding protein interactions in real-time, effortlessly, leading you in no time to highly impactful discoveries.