13 – 14 June 2023

Edition 2: Experimental design and data analysis

Join this 1.5 day workshop to learn how to best design your DNA-protein interaction experiments with the C-Trap and how to extract the relevant information from your data.
Learn key biophysical concepts to best design your next single-molecule experiments
Get support from in-house experts and fellow users

About this class

In this class, you will be introduced to biophysical concepts relevant to DNA-protein interaction experiments. This includes binding kinetics, protein stoichiometry, protein mobility, single-molecule statistics, and more. Using these concepts, you will be able to refine your experimental approach, maximize the quality of your fluorescence and force data and subsequently get the most out of them. Each learning will be put to practice through real C-Trap data analysis using Lakeview and Pylake, and through open discussions on conceptual experimental design.

About your teachers

Vincenzo Mascoli studied Chemistry at the University of Pisa, where he got a master degree in 2016. That same year, he moved to Amsterdam for his PhD in the group of prof. Croce, applying ultrafast spectroscopy techniques to study light harvesting in plants and photosynthetic bacteria. He obtained his PhD in biophysics in 2021 and, a few months later, he joined LUMICKS as an Application Scientist. In his role, he has spent a huge amount of time with the C-Trap system, performing demonstrations and developing new assays. Following his passion for customer support and business development, he has also started taking care of projects in Southern Europe. Besides being a pro-active scientist, he is also passionate about tennis and pop music and sings in male choir in Amsterdam.

Aafke van den Berg obtained her degree in Applied Physics at Delft University of Technology. She entered the field of Biophysics as a research assistant in the lab of Nynke Dekker, using magnetic tweezers. Excited by the fundamental questions that can be addressed using single-molecule techniques, she decided to do her PhD in the Depken lab. There she analyzed single-molecule fluorescence data on replication and modelled the interactions between nucleosomes and transcription. She then transitioned to a postdoc in the Mirny lab at MIT. There she combined computational modelling and data analysis to unravel the interactions between loop extrusion and transcription. Aafke is currently a Sr Customer Success Data Scientist in the LEC team and supports customers in data analysis.

Agenda

Day 1 – Tuesday 13 June

11:30 – 12:30    C-Trap MasterClass kickoff
12:30 – 13:30    Lunch
13:30 – 15:30    Quantifying protein binding
15:30 – 16:00    Coffee break
16:00 – 17:30    Binding location and diffusion analysis
17.30 – 19.00    Free time
19:00 – 21:30    Dinner

Day 2 – Wednesday 14 June

09:00 – 09:30    Coffee
09:30 – 11:00    Analyzing force-extension curves
11.00 – 11:15    Short break
11.15 – 12:15    Quantifying active protein translocation
12:15 – 13:15    Lunch
13:15 – 14:30    Open Q&A with experts
14:30 – 15:00    Break
15:00 – 16:00    Statistics + Tips and tricks
16:00 – 16:15    Short break
16:15 – 17:00    Feedback and closing

I want to attend this MasterClass! What should I know?

  • This event costs 275 euros, which includes, coffee, snacks, two lunches, and one dinner
  • The MasterClass will take place at our Amsterdam headquarters
  • Travel and accommodation are NOT included
  • This MasterClass class consists of lectures, open discussions and data analysis sessions
  • The start and end time of each day will remain fixed but the final daily content may var

Registration is closed

Thank you for your interest! The current class is full. If you would like to attend future MasterClasses, please reach out to your corresponding Application Scientist to express your interest. Until next time!