Cytoskeletal Motors

Part of: Cellular Structure and Transport

Today’s scientific trends are racing towards smaller scales and experimentation that provides both structural and mechanistic insights. To decipher the activity and mechanical properties of cellular components you need methods that combine visualization capabilities with force measurements and manipulation. We offer solutions that enable you to measure, manipulate and visualize individual filaments and their motors in real-time, with both high throughput and resolution.

Scientists can use optical tweezers to trap beads and catch a filament with motor proteins bound to it in-between. This filament can then be manipulated by moving the beads, while the force and extension are measured. Combining optical tweezers with simultaneous fluorescence measurements allows visualizing the binding location of fluorescently-tagged motors along the filaments. With optical tweezers – fluorescence microscopy you can:


  • Visualize and track the kinetics of molecular motors along cytoskeletal proteins and determine their activity, processive motion, binding kinetics and thermodynamic properties
  • Investigate discrete steps and the subsequent mechanics of motor proteins at the single-molecule level
  • Perform experiments under biologically relevant conditions and highly crowded environments and link the in vitro experiment with the in vivo situation
  • Study motor motion at different ATP, salt, small molecule and biologics concentrations within a single experiment

Learn more about:
Technology
Optical Tweezers and Fluorescence Microscopy
Optical Tweezers and Fluorescence Microscopy

Technology

The combination of optical tweezers and fluorescence microscopy allows for simultaneous manipulation and visualization of molecular interactions in real-time

Solutions

C-Trap Optical Tweezers Fluorescence Microscopy
C-Trap™

Optical Tweezers and Fluorescence Microscopy

m-Trap™ Optical Tweezers
m-Trap™

Optical Tweezers

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