Cytoskeletal Motors

Part of: Cellular Structure and Transport

Today’s scientific trends are racing towards smaller scales and experimentation that provides both structural and mechanistic insights. To decipher the activity and mechanical properties of cellular components you need methods that combine visualization capabilities with force measurements and manipulation. We offer solutions that enable you to measure, manipulate and visualize individual filaments and their motors in real-time, with both high throughput and resolution.

Scientists can use optical tweezers to trap beads and catch a filament with motor proteins bound to it in-between. This filament can then be manipulated by moving the beads, while the force and extension are measured. Combining optical tweezers with simultaneous fluorescence measurements allows visualizing the binding location of fluorescently-tagged motors along the filaments. With optical tweezers – fluorescence microscopy you can:


  • Visualize and measure molecular motion with unprecedented resolution: determine the activity, velocity, binding kinetics, frequency, and run length of both non-processive and processive motors.
  • Study motor activity and (un)binding with zero perturbation and perform mechanochemical studies under controlled external loads with sub-nanometer resolution and sub-picoNewton force sensitivity.
  • Investigate discrete steps and the subsequent mechanics of motor proteins at the single-molecule level
  • Perform experiments under biologically relevant conditions and highly crowded environments and link the in vitro experiment with the in vivo situation
  • Study motor motion at different ATP, salt, small molecule and biologics concentrations within a single experiment

Learn more about:
Technology
Optical Tweezers and Fluorescence Microscopy
Optical Tweezers and Fluorescence Microscopy

Technology

The combination of optical tweezers and fluorescence microscopy allows for simultaneous manipulation and visualization of molecular interactions in real-time

Solutions

C-Trap

Optical Tweezers and Fluorescence Microscopy

m-Trap

Optical Tweezers and Fluorescence Microscopy

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