Cytoskeletal Filaments

Part of: Cellular Structure and Transport

Today’s scientific trends are racing towards smaller scales and experimentation that provides both structural and mechanistic insights. To decipher the activity and mechanical properties of cellular components you need methods that combine visualization capabilities with force measurements and manipulation. We offer solutions that enable you to measure, manipulate and visualize individual filaments and their motors in real-time, with both high throughput and resolution.

Scientists can use optical tweezers to trap beads, as depicted at the right, and catch a filament in between. This filament can then be manipulated by moving the beads, while the force and extension are measured. Combining optical tweezers with simultaneous fluorescence measurements allows correlating the mechanical properties of the microtubule with local information. With optical tweezers – fluorescence microscopy you can:


  • Directly measure the mechanical properties of filaments and manipulate them to find their thresholds and energy dissipation mechanisms
  • Correlate global mechanical properties of the filament with local structural information
  • Observe and correlate the effect of binding and motion of single molecules on filament mechanics and dynamics
  • Visualize and measure filament-filament, filament-protein, and filament-protein-filament interactions
  • Perform experiments under biologically relevant conditions and highly crowded environments and link the in vitro experiment with the in vivo situation
  • Study the effect of small molecules and biologics in filament structure and dynamics.

Learn more about:
Technology
Optical Tweezers and Fluorescence Microscopy
Optical Tweezers and Fluorescence Microscopy

Technology

The combination of optical tweezers and fluorescence microscopy allows for simultaneous manipulation and visualization of molecular interactions in real-time

Solutions

C-Trap Optical Tweezers Fluorescence Microscopy
C-Trap™

Optical Tweezers and Fluorescence Microscopy

m-Trap™ Optical Tweezers
m-Trap™

Optical Tweezers

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