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C-Trap DNA Organization

Study and visualize DNA organization mechanisms at the nanoscale

Motor packaging activity and stepping behavior with base pair resolution Here, we tethered a φ29 DNA packaging motor and its dsDNA substrate between two optically trapped beads. Using optical tweezers we can perform pulling and equilibrium experiments to probe the structural dynamics and conformational changes during genome packaging.As packaging proceeds, the DNA…
getyos
9 December 2020
C-Trap RNA Mechanics

Study and visualize DNA / RNA structural dynamics at the nanoscale

Force extension, manipulation, and visualization of DNA overstretching Here, a single double-stranded DNA (dsDNA) molecule is tethered between two beads and incubated with Sytox Orange, a dsDNA fluorescent marker, and fluorescently labeled replication protein A (RPA), an ssDNA-binding replication protein.Figure 1 shows a dsDNA molecule being stretched with a constant…
getyos
9 December 2020
C-Trap Protein Folding

Study protein folding and conformational dynamics at the nanoscale

Study of multi-domain protein folding & conformational dynamics In this experiment, a protein is tethered between two optically trapped beads. The folding and unfolding of the protein is controlled by moving the beads while the force and distance are measured simultaneously.The resulting force-distance curve (Figure 1) of calmodulin – a…
getyos
8 December 2020
C Trap Co translational Step 1

Study and visualize RNA translation mechanisms at the nanoscale

Visualization of DNA-protein interactions In this experiment, a DNA molecule is tethered between two beads while multiple fluorescently labeled proteins are interacting with it. We can visualize these interactions and track them over time using multicolor confocal or STED fluorescence microscopy. The resulting kymograph unveils the number, position, diffusion, and…
getyos
8 December 2020
C-Trap Filaments

Study cytoskeletal filaments in real-time at the nanoscale

Force-extension, manipulation, and visualization of polymers and protein filaments In this experiment, a vimentin filament is tethered between two optically trapped beads. We can obtain the force-distance curve and determine the mechanical properties of the microtubule by simultaneously stretching the filament and measuring the force and the extension.The measured data in…
getyos
8 December 2020
C-Trap Motor Visualization

Study cytoskeletal motors in real-time at the nanoscale

Single-molecule visualization of cytoskeletal motors in solution using confocal or STED In this experiment a microtubule is tethered between two optically trapped beads with fluorescently-labeled motor proteins bound to it. We can uncover the binding location, (un)binding events and kinetics of the cytoskeletal motors by keeping the microtubule stretched and…
getyos
8 December 2020
Cell receptor responses_C Trap_optical tweezers fluorescence microscopy_experiment

Manipulate and visualize cellular functions at nanoscale and in real time

Receptor responses to mechanical stimuli   In this experiment, we used the C-Trap to understand the properties involved in the mechanical activation of transmembrane receptors. While applying a controlled mechanical stimulus, we monitored the activation of intracellular responses and signals in real time.We guided a microscopic bead to the cell…
getyos
8 December 2020

Study protein droplet dynamics in real time to understand phase separation

Variations in low-complexity domains and how they influence protein droplet solidification Many peptides or proteins involved in phase separation contain low-complexity domains that can induce protein and amyloid aggregates, involved in some neurodegenerative diseases. Here, researchers from the lab of Dr. Bo Sun at ShanghaiTech used the C-Trap to assess how…
getyos
8 December 2020

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